Novel approach for evaluation of estrogenic and anti-estrogenic activities of genistein and daidzein using B16 melanoma cells and dendricity assay

Pigment Cell Research
Kouji Miyazaki

Abstract

The effects of soy isoflavones, genistein and daidzein, which exhibit estrogenic, anti-estrogenic and/or tyrosine kinase inhibitory activity, on the dendritic morphology of B16 mouse melanoma cells were quantitatively evaluated and compared with those of 17 beta-estradiol (Est) and tyrphostin, a tyrosine kinase inhibitor. Dendricity was significantly stimulated in the order of Est > genistein > daidzein = tyrphostin, but not by glycosides of genistein and daidzein. In competition experiments, Est counteracted the stimulatory activity of genistein and daidzein, but enhanced the activity of tyrphostin additively, suggesting that genistein and daidzein agonized Est. In addition, when the concentration ratios of genistein/Est and daidzein/Est were higher than 5000 and 50,000, respectively, genistein and daidzein agonized Est. In contrast, when the ratio of daidzein/Est was lower than 500, daidzein antagonized Est. Furthermore, genistein and daidzein competed with each other in stimulatory activity. These observations suggest that: 1) dendricity is stimulated by agonists (genistein and daidzein) of Est and tyrosine kinase inhibitors (genistein and tyrphostin), 2) the concentration ratio of isoflavone aglycone/Est is very important a...Continue Reading

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Citations

Oct 22, 2008·Proceedings of the National Academy of Sciences of the United States of America·Junji MorokumaMichael Levin
May 3, 2011·Biological & Pharmaceutical Bulletin·Eun-Jung ChoiJae-Kwan Hwang
Jun 14, 2005·Expert Opinion on Therapeutic Targets·M J Thornton
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Nov 12, 2016·Frontiers in Endocrinology·Monica MarzagalliPatrizia Limonta
Sep 13, 2020·European Journal of Clinical Nutrition·Chisato Nagata
Aug 3, 2021·Oxidative Medicine and Cellular Longevity·Javad Sharifi-RadDaniela Calina

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