Novel approach reveals localisation and assembly pathway of the PsbS and PsbW proteins into the photosystem II dimer

FEBS Letters
Ellinor ThidholmChristiane Funk

Abstract

A blue-native gel electrophoresis system was combined with an in organello import assay to specifically analyse the location and assembly of two nuclear-encoded photosystem II (PSII) subunits. With this method we were able to show that initially the low molecular mass PsbW protein is not associated with the monomeric form of PSII. Instead a proportion of newly imported PsbW is directly assembled in dimeric PSII supercomplexes with very fast kinetics; its negatively charged N-terminal domain is essential for this process. The chlorophyll-binding PsbS protein, which is involved in energy dissipation, is first detected in the monomeric PSII subcomplexes, and only at later time points in the dimeric form of PSII. It seems to be bound tighter to the PSII core complex than to light harvesting complex II. These data point to radically different assembly pathways for different PSII subunits.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Nov 15, 1981·Analytical Biochemistry·W WrayR Hancock
Jul 21, 1995·The Journal of Biological Chemistry·K D IrrgangW P Schröder
Dec 30, 1994·Biochimica Et Biophysica Acta·S KimC F Yocum
Jan 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·E J BoekemaM Rögner
Jun 20, 1998·The Journal of Biological Chemistry·D ZhelevaJ Barber
Mar 24, 1999·Trends in Biochemical Sciences·R E Dalbey, C Robinson
Mar 31, 1999·Trends in Biochemical Sciences·J BarberB Hankamer
Aug 17, 1999·Current Opinion in Structural Biology·J Barber, W Kühlbrandt
Nov 15, 2000·Biological Chemistry·U C Vothknecht, J Soll
Dec 29, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·J NieldJ Barber
Dec 29, 2000·Nature·UNKNOWN Arabidopsis Genome Initiative
May 1, 1980·Plant Physiology·J E MulletC J Arntzen

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Citations

Sep 7, 2005·Photosynthesis Research·Jun Minagawa, Yuichiro Takahashi
Apr 14, 2011·Photosynthesis Research·Cristina PaglianoJames Barber
Jul 11, 2006·Plant Physiology·Mounia HeddadIwona Adamska
Nov 17, 2005·Plant Methods·Holger EubelA Harvey Millar
Dec 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Elisabetta BergantinoIldikò Szabò
Sep 14, 2006·Photosynthesis Research·Sofía AndaluzAnunciación Abadía
Aug 22, 2009·The EMBO Journal·Stefano CaffarriRoberta Croce
Sep 26, 2007·Proteomics·Veronika Reisinger, Lutz Andreas Eichacker
Jan 27, 2011·The Plant Journal : for Cell and Molecular Biology·José G García-CerdánWolfgang P Schröder
Mar 23, 2011·The Plant Journal : for Cell and Molecular Biology·Roman LadigEnrico Schleiff
Sep 23, 2008·Analytical Biochemistry·Bernhard GranvoglLutz Andreas Eichacker
Feb 12, 2004·Biochimica Et Biophysica Acta·Lan-Xin Shi, Wolfgang P Schröder
Sep 13, 2011·Biochimica Et Biophysica Acta·Lan-Xin ShiWolfgang P Schröder
Sep 25, 2012·Biochimica Et Biophysica Acta·Thomas Roach, Anja Krieger-Liszkay
Dec 29, 2004·Biochimica Et Biophysica Acta·Jan P Dekker, Egbert J Boekema
Jun 1, 2016·Frontiers in Plant Science·Daniel A WeiszHimadri B Pakrasi
Oct 5, 2016·Scientific Reports·Jarosław KróliczewskiBożena Króliczewska
Oct 11, 2016·Molecular Genetics and Genomics : MGG·Longxin WangDeqiang Zhang
Nov 16, 2006·The Journal of Biological Chemistry·Danny YaoChristiane Funk
Sep 29, 2004·The Journal of Biological Chemistry·Lutz A EichackerEnrico Schleiff
Sep 14, 2019·Biochimica Et Biophysica Acta. Bioenergetics·Haijun Liu, Robert E Blankenship
Nov 8, 2006·Biochemistry·Cécile BreytonGiovanni Finazzi
Feb 1, 2005·Proteomics·Corrado CiambellaLello Zolla

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