Novel approaches to study coronary vasculature development in mice

Developmental Dynamics : an Official Publication of the American Association of Anatomists
Hana KolesováDavid Sedmera

Abstract

Coronary artery development is an intensely studied field. Mice are a popular genetic model for developmental studies, but there is no widely accepted protocol for high-throughput, high-resolution imaging of their developmental and adult coronary artery anatomy. Using tissue-clearing protocols and confocal microscopy, we have analyzed embryonic and juvenile mouse hearts in Cx40:GFP knock-in models with a special focus on septal artery development. We found that the septal artery, which supplies the interventricular septum, was initially formed as an arterial plexus that connected to both the left and right coronary arteries. During development, the plexus was remodeled into a single tube, which then remained connected only to the right coronary artery. Since optical imaging became limited at postnatal stages, it was supplemented with injection techniques using India ink and Microfil; the latter was subsequently analyzed with micro-CT to visualize the anatomy of coronary vessels in 3D. The techniques described here enable us to study the finer details of coronary artery development in mice and can, therefore, be implemented to study the pathogenesis of coronary malformations in various mouse models. Developmental Dynamics 247:10...Continue Reading

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Citations

Jun 15, 2019·Proceedings of the National Academy of Sciences of the United States of America·Romana BohuslavovaGabriela Pavlinkova
Sep 13, 2020·Scientific Reports·Maryse Lapierre-LandryMichael W Jenkins
Jul 25, 2019·Progress in Biophysics and Molecular Biology·Anna Di BonaTania Zaglia
Mar 21, 2021·Current Opinion in Hematology·Pamela E Rios Coronado, Kristy Red-Horse
May 14, 2021·IScience·Hana KolesováDavid Sedmera
Sep 11, 2021·Vascular Pharmacology·Jingtan ZhuDan Zhu
Oct 2, 2021·Development·Alba Vieites-Prado, Nicolas Renier

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