Novel aspartyl proteinase associated to fat body histolysis during Ceratitis capitata early metamorphosis

Archives of Insect Biochemistry and Physiology
Alejandro RabossiLuis A Quesada-Allué

Abstract

During larva to adult transition, the larval fat body of the Medfly (Ceratitis capitata) progressively disintegrates to be replaced by the adult one, after imago ecdysis. Here we show that a temporal correlation exists among the microscopy images of fat body progressive disintegration, the activation of fat body lysosomes (as judged by acid phosphatase activity), and the activity of a novel fat body aspartyl proteinase. The enzyme was purified and partially characterized. This proteinase exhibited a wide range of acid isoforms with isoelectric points from 5.6 to 7.3, an optimum pH of 3.0 for hemoglobin digestion, and was completely inhibited by pepstatin A. The apparent molecular weight was estimated (42 +/- 1 kDa) and the protein was characterized as N-glycosylated, judging from affinity to Concanavalin A. From the biochemical characteristics, the enzyme that we called "Early Metamorphosis Aspartyl Proteinase" (EMAP) appears to be similar to mammalian Cathepsin D. However, the N-terminal sequence of EMAP showed no similarity with any known animal Cathepsins and exhibited an important instability to neutral and alkaline pH. This feature seems to be a peculiar characteristic of insect aspartyl proteinases. The temporal activity ...Continue Reading

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Citations

Mar 6, 2010·Photochemistry and Photobiology·Luciana Mercedes Pujol-LereisLuis Alberto Quesada-Allué
Oct 26, 2006·BMC Developmental Biology·Zhong Zheng GuiByung Rae Jin
Jul 27, 2007·BMC Developmental Biology·Shiping LiuZhonghuai Xiang
Jul 26, 2012·Biochemical and Biophysical Research Communications·Jie YuZhong-Zheng Gui
Jun 23, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Kwang Sik LeeByung Rae Jin
May 19, 2009·Journal of Insect Physiology·Ji-Eun Ahn, Keyan Zhu-Salzman
Oct 28, 2008·Experimental Parasitology·Rodrigo da Rocha FragosoMaria Fátima Grossi-de-Sa
Jan 1, 2008·Archives of Insect Biochemistry and Physiology·Alejandro RabossiLuis A Quesada-Allué
Apr 8, 2015·The International Journal of Biochemistry & Cell Biology·Zheng WangGuohua Zhong
Jul 27, 2006·The New Phytologist·William E Finch-Savage, Gerhard Leubner-Metzger
Jan 9, 2014·Molecular Biology Reports·Jie YuZhong-Zheng Gui

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