Novel Assessment of Urinary Albumin Excretion in Type 2 Diabetes Patients by Raman Spectroscopy

Diagnostics
Jose L Flores-GuerreroJorge Castro-Ramos

Abstract

. Urinary albumin excretion remains the key biomarker to detect renal complications in type 2 diabetes. As diabetes epidemy increases, particularly in low-income countries, efficient and low-cost methods to measure urinary albumin are needed. In this pilot study, we evaluated the performance of Raman spectroscopy in the assessment of urinary albumin in patients with type 2 diabetes. The spectral Raman analysis of albumin was performed using artificial urine, at five concentrations of albumin and 24 h collection urine samples from ten patients with Type 2 Diabetes. The spectra were obtained after removing the background fluorescence and fitting Gaussian curves to spectral regions containing features of such metabolites. In the samples from patients with type 2 diabetes, we identified the presence of albumin in the peaks of the spectrum located at 663.07, 993.43, 1021.43, 1235.28, 1429.91 and 1633.91 cm-1. In artificial urine, there was an increase in the intensity of the Raman signal at 1450 cm-1, which corresponds to the increment of the concentrations of albumin. The highest concentration of albumin was located at 1630 cm-1. The capability of Raman spectroscopy for detection of small concentrations of urinary albumin suggests ...Continue Reading

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Citations

May 15, 2021·Applied Spectroscopy·Nicole M Ralbovsky, Igor K Lednev
Jan 13, 2021··C. Villalba-HernándezF. Gutiérrez-Delgado

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Methods Mentioned

BETA
enzyme-
linked immunosorbent assays
Raman
Raman spectroscopy
urine collection

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