Novel autoantigens in autoimmune hypophysitis

Clinical Endocrinology
Isabella LupiPatrizio Caturegli

Abstract

Pituitary autoantibodies are found in autoimmune hypophysitis and other conditions. They are a marker of pituitary autoimmunity but currently have limited clinical value. The methods used for their detection lack adequate sensitivity and specificity, mainly because the pathogenic pituitary autoantigen(s) are not known and therefore antigen-based immunoassays have not been developed. This study aimed to identify novel pituitary autoantigens using sera as probes in proteomic assays. We also compared immunoblotting and immunofluorescence methods for their accuracy in diagnosing autoimmune hypophysitis. Twenty-eight sera from autoimmune hypophysitis cases (14 histologically proven and 14 clinically suspected) were compared to 98 sera from controls, which included 14 patients with pituitary adenomas, 48 with autoimmune thyroiditis (15 Graves' disease and 33 Hashimoto's thyroiditis) and 36 healthy subjects. All sera were tested against human pituitary cytosolic proteins separated by one-dimensional (1D) gel electrophoresis. The band recognition was analysed statistically to detect molecular weight regions preferentially recognized by hypophysitis sera. 2D gel immunoblotting and mass spectrometry were then used to sequence the protein...Continue Reading

References

Nov 1, 1970·The Journal of Pathology·J S Beck, J M Melvin
Jan 24, 2002·Hormone Research·T TakaoK Hashimoto
Feb 21, 2002·Journal of Molecular Endocrinology·S TanakaN Amino
Mar 16, 2002·Nature Immunology·Isao MatsumotoChristophe Benoist
Oct 18, 2002·Journal of Cancer Research and Clinical Oncology·Jianhua Wang, Shishu Chen
Nov 30, 2002·European Journal of Endocrinology·Susumu TanakaNobuyuki Amino
Feb 8, 2003·The Journal of Clinical Endocrinology and Metabolism·Annamaria De BellisAntonio Bellastella
Jan 8, 2004·Endocrine Journal·Susumu TanakaNobuyuki Amino
Jun 25, 2004·The Protein Journal·Daniel HirschbergHans Jörnvall
Aug 18, 2004·Journal of Neurosurgery·Gilberto K K LeungEdward R Laws
Jun 1, 1993·Current Biology : CB·D J PappinA J Bleasby
Nov 25, 2004·Molecular & Cellular Proteomics : MCP·Guido SauerHerman H W Silljé
Jan 7, 2005·Endocrine Reviews·Patrizio CaturegliNoel R Rose
Jan 7, 2005·Nature·Jens S AndersenMatthias Mann
Jul 18, 2006·Journal of Neuroimmunology·Chunhe WangArthur A Vandenbark
Nov 9, 2006·The Journal of Clinical Endocrinology and Metabolism·Annamaria De BellisAntonio Bellastella
Mar 8, 2007·The Journal of Clinical Endocrinology and Metabolism·Luca ManettiEnio Martino
Apr 12, 2007·Clinical Reviews in Allergy & Immunology·Miri BlankYehuda Shoenfeld
Jun 8, 2007·The Journal of Clinical Endocrinology and Metabolism·Patrizio Caturegli
Jun 15, 2007·Nature·Shalina S OusmanLawrence Steinman
Aug 19, 2007·Cell·Jürgen Cox, Matthias Mann
Oct 19, 2007·Proceedings of the National Academy of Sciences of the United States of America·Janet M WenzlauJohn C Hutton

❮ Previous
Next ❯

Citations

May 26, 2009·Pituitary·Angelika GutenbergPatrizio Caturegli
Oct 18, 2011·Pituitary·Federica GuaraldiRoberto Salvatori
Mar 20, 2012·Endocrine·A Glezer, M D Bronstein
Mar 14, 2013·Journal of Endocrinological Investigation·I LupiE Martino
Aug 31, 2013·Pituitary·Benjamin H M HunnCatriona A Mclean
Aug 5, 2015·Journal of Clinical Medicine·Federica GuaraldiEzio Ghigo
Nov 26, 2015·European Journal of Endocrinology·Annamaria De BellisUNKNOWN Italian Autoimmune Hypophysitis Network Group
Nov 1, 2012·Annales d'endocrinologie·I Allix, V Rohmer
Jun 23, 2009·Autoimmunity Reviews·Melissa A Landek-SalgadoPatrizio Caturegli
Sep 9, 2008·Autoimmunity Reviews·Patrizio CaturegliNoel R Rose
Jan 1, 2014·Journal of Clinical Laboratory Analysis·Rocío AguadoConsuelo Ortega
May 13, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Lin YangYong Chen
Aug 12, 2014·La Revue de médecine interne·I Allix, V Rohmer
Apr 23, 2016·Der Pathologe·W Saeger
Oct 31, 2015·Annales d'endocrinologie·Ingrid Allix, Vincent Rohmer
Nov 4, 2017·International Journal of Molecular Sciences·Cristina CoccoGian-Luca Ferri
Jun 9, 2018·European Journal of Endocrinology·Mamta N JoshiPaul V Carroll
Jan 12, 2010·Endocrine Practice : Official Journal of the American College of Endocrinology and the American Association of Clinical Endocrinologists·Charalambos DemetriJ Enrique Silva
Sep 1, 2014·Expert Review of Endocrinology & Metabolism·Annamaria De BellisKatherine Esposito
Apr 29, 2015·The Journal of Clinical Endocrinology and Metabolism·Shintaro IwamaYutaka Oiso
Dec 22, 2016·Molecular Medicine Reports·Yan-Wu ZhouChun-Fang Zhang
Feb 26, 2020·International Journal of Molecular Sciences·Giuseppe BellastellaAnnamaria De Bellis
Jul 14, 2017·Clinical Diabetes and Endocrinology·Alexander Faje
Mar 14, 2019·Archives of Endocrinology and Metabolism·Narin Nasiroglu ImgaDilek Berker
Sep 1, 2021·Expert Review of Clinical Immunology·Francesco FrascaRosaria Maddalena Ruggeri

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.

Autoimmune Thyroiditis

Autoimmune thyroiditis is an inflammatory disease of thyroid gland due to autoimmune responses leading to lymphocytic infiltration of the gland. It is characterized by the presence of circulating thyroid antigen-specific T-cells and thyroid autoantibodies. Discover the latest research on autoimmune thyroiditis here.