PMID: 9420277Jan 7, 1998Paper

Novel binding sites for regulatory factors in the human papillomavirus type 18 enhancer and promoter identified by in vivo footprinting

Journal of Virology
P H BednarekB Phillips

Abstract

The E6 and E7 genes of human papillomaviruses (HPVs) associated with anogenital cancers are largely responsible for the oncogenic activity of these viruses, and regulation of these genes has been intensively studied. Transcription of the E6 and E7 genes is controlled by the viral upstream regulatory region (URR). We have used in vivo footprinting to examine the occupancy by regulatory factors of the HPV type 18 (HPV18) URR enhancer and promoter in the cervical carcinoma cell lines HeLa and C4-II. While corroborating occupancy in vivo of all of the elements previously implicated in the transcriptional control of the HPV18 E6 and E7 genes by in vitro DNase I footprinting, gel retardation assays, and transfection studies, we also detect occupancy in vivo of several enhancer and promoter sequences which have not been previously identified as HPV18 URR regulatory elements. Our data suggest that the HPV18 enhancer and promoter are more densely occupied by DNA-binding proteins than previously thought and raise the possibility that additional, possibly novel factors contribute to transcription of the HPV18 early genes.

References

May 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J D McGhee, G Felsenfeld
Feb 1, 1992·Human Pathology·M H StolerT R Broker
May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·D V HeckK Münger
Oct 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D H Mack, L A Laimins
Feb 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M von Knebel DoeberitzH zur Hausen
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·S Jeon, P F Lambert
Aug 1, 1994·Molecular and Cellular Biology·S P MurphyB Phillips
Jul 1, 1994·Molecular Carcinogenesis·F Hoppe-Seyler, K Butz
Sep 1, 1993·Analytical Biochemistry·I K Hornstra, T P Yang

❮ Previous
Next ❯

Citations

Oct 23, 2002·The Journal of Experimental Medicine·Edgar GrinsteinHans-Dieter Royer
Mar 18, 2003·Molecular and Cellular Biology·Isabelle BouallagaFrançoise Thierry

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.