Novel biological activity of ameloblastin in enamel matrix derivative

Journal of Applied Oral Science : Revista FOB
Sachiko Kuramitsu-FujimotoTatsuji Nishihara

Abstract

Enamel matrix derivative (EMD) is used clinically to promote periodontal tissue regeneration. However, the effects of EMD on gingival epithelial cells during regeneration of periodontal tissues are unclear. In this in vitro study, we purified ameloblastin from EMD and investigated its biological effects on epithelial cells. Bioactive fractions were purified from EMD by reversed-phase high-performance liquid chromatography using hydrophobic support with a C18 column. The mouse gingival epithelial cell line GE-1 and human oral squamous cell carcinoma line SCC-25 were treated with purified EMD fraction, and cell survival was assessed with a WST-1 assay. To identify the proteins in bioactive fractions of EMD, we used proteome analysis with two-dimensional gel electrophoresis followed by identification with liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Purified fractions from EMD suppressed proliferation of GE-1 and SCC-25. LC-MS/MS revealed that ameloblastin in EMD is the component responsible for inhibiting epithelial cell proliferation. The inhibitory effect of ameloblastin on the proliferation of GE-1 and SCC-25 was confirmed using recombinant protein. The inhibitory effects of EMD on epithelial cell prolif...Continue Reading

References

Feb 23, 1996·The Journal of Biological Chemistry·P H KrebsbachY Yamada
Jul 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·R CernýT Wurtz
Oct 6, 1997·Journal of Clinical Periodontology·L Hammarström
Oct 6, 1997·Journal of Clinical Periodontology·S GestreliusL Hammarström
Oct 6, 1997·Journal of Clinical Periodontology·S GestreliusM Somerman
Feb 22, 2002·Journal of Biomedical Materials Research·Miho Shimizu-IshiuraTakahisa Sasaki
Apr 25, 2003·Journal of Periodontology·D T Graves, D Cochran
May 11, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·HongJun WangMichael L Paine
Sep 1, 2005·Journal of Clinical Periodontology·P N MadianosD F Kinane
Jul 11, 2006·Journal of Periodontal Research·N ItohT Nishihara
Dec 13, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Catherine Fenselau
Jan 5, 2007·Briefings in Functional Genomics & Proteomics·Benito CañasEnrique Calvo
Aug 19, 2007·European Journal of Oral Sciences·Nazanin NaraniHannu Larjava
Sep 14, 2010·European Journal of Oral Sciences·Margareth V TamburstuenJanne E Reseland
Nov 11, 2011·Tissue Engineering. Part B, Reviews·H Michelle GrandinMichel Dard
Nov 14, 2013·Clinical Oral Investigations·Reinhard GruberAnton Sculean
May 16, 2014·Journal of Periodontal Research·R GruberA Sculean
May 27, 2014·Archives of Oral Biology·Noriko SaitoTatsuji Nishihara
Jun 17, 2014·PloS One·Jaime JacquesJuliane Isaac

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Citations

Jun 18, 2016·Journal of Periodontology·Sabine GroegerJoerg Meyle
Jan 27, 2017·Experimental and Therapeutic Medicine·Marzena Wyganowska-SwiatkowskaMalgorzata Kotwicka

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Methods Mentioned

BETA
protein assay
electrophoresis

Software Mentioned

MASCOT
JMP

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