Novel biological properties of Oenothera paradoxa defatted seed extracts: effects on metallopeptidase activity

Journal of Agricultural and Food Chemistry
Anna K KissMarek Naruszewicz

Abstract

In this study, for the first time, we used the in vitro metallopeptidase model for the identification of a potential novel activity of defatted evening primrose seed extracts. Prepared extracts of different polarity (aqueous, 60% ethanolic, isopropanolic, and 30% isopropanolic) at concentrations of 1.5-100 microg/mL exhibited a significant and dose dependent inhibition of three tested enzymes. The 50% inhibition of enzymes activity showed that aminopeptidase N (APN) was the enzyme affected to the greatest extent with IC50 at the level of 2.8 microg/mL and 2.9 microg/mL for aqueous and 30% isopropanolic extracts, respectively. The activity of neutral endopeptidase (NEP) was quite strongly inhibited by the extracts as well. The HPLC-DAD analysis and bioguided fractionation led to the identification of four active compounds: (-)-epicatechin gallate, proanthocyanidin B3, oenothein B, and penta-O-galloyl-beta-D-glucose (PGG). Oenothein B has been shown previously to inhibit metallopeptidases. The three other compounds are known to inhibit angiotensin-converting enzyme (ACE), but they have not been previously reported to inhibit the NEP and APN activity. PGG and procyanidins with different degrees of polymerization, as the dominating...Continue Reading

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Citations

Jul 31, 2013·Journal of Photochemistry and Photobiology. B, Biology·Edyta JaszewskaMarek Naruszewicz
Jun 2, 2011·Journal of Agricultural and Food Chemistry·Sylwia GorlachMaria Koziołkiewicz
Feb 18, 2014·Journal of Natural Medicines·Yanyan CaoXiaozhuo Chen
Dec 15, 2015·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Sebastian GranicaAnna K Kiss
Mar 20, 2020·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Denisa SkarupovaAlena Rajnochova Svobodova
Aug 8, 2021·International Journal of Molecular Sciences·Malgorzata Chmielewska-Kassassir, Lucyna A Wozniak

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