Novel biomarkers distinguishing active tuberculosis from latent infection identified by gene expression profile of peripheral blood mononuclear cells

PloS One
Chanyi LuWenhong Zhang


Humans infected with Mycobacterium tuberculosis (MTB) can delete the pathogen or otherwise become latent infection or active disease. However, the factors influencing the pathogen clearance and disease progression from latent infection are poorly understood. This study attempted to use a genome-wide transcriptome approach to identify immune factors associated with MTB infection and novel biomarkers that can distinguish active disease from latent infection. Using microarray analysis, we comprehensively determined the transcriptional difference in purified protein derivative (PPD) stimulated peripheral blood mononuclear cells (PBMCs) in 12 individuals divided into three groups: TB patients (TB), latent TB infection individuals (LTBI) and healthy controls (HC) (n = 4 per group). A transcriptional profiling of 506 differentially expressed genes could correctly group study individuals into three clusters. Moreover, 55- and 229-transcript signatures for tuberculosis infection (TB&LTBI) and active disease (TB) were identified, respectively. The validation study by quantitative real-time PCR (qPCR) performed in 83 individuals confirmed the expression patterns of 81% of the microarray identified genes. Decision tree analysis indicated t...Continue Reading


Aug 1, 1985·The Journal of Clinical Investigation·T UchiyamaH Uchino
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·Carol BeadlingK A Smith
Jul 11, 1998·The Journal of Biological Chemistry·Y WengJ A DeMartino
Dec 9, 2000·American Journal of Respiratory and Critical Care Medicine·S M MarksC H Nguyen
Feb 13, 2001·European Journal of Immunology·L A van PinxterenP Andersen
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Aug 14, 2002·Biochemistry·Valerie BoothBrian D Sykes
Dec 3, 2002·Medical Immunology·Carol Beadling, Kendall A Smith
Mar 31, 2004·The Journal of Nutrition·Madhu S DharLawrence W Castellani
Dec 2, 2004·The European Respiratory Journal·P-J Cardona, J Ruiz-Manzano
Dec 15, 2004·Microbes and Infection·Chandrashekhar Pasare, Ruslan Medzhitov
Jun 4, 2005·Advances in Experimental Medicine and Biology·Chandrashekhar Pasare, Ruslan Medzhitov
Feb 24, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jung-Su ChangG A W Rook
Jan 6, 2007·The Journal of Infectious Diseases·Rohit MistryPauline T Lukey
Feb 24, 2007·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Marc JacobsenS H E Kaufmann
Mar 7, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Bo WuCarol Clayberger
Apr 21, 2007·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Joel Fleury Djoba SiawayaG Walzl
Nov 22, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Man-Fung YuenChing-Lung Lai
Aug 8, 2008·The European Respiratory Journal·M RuhwaldTBNET
Nov 21, 2008·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·C B E CheeY T Wang
Dec 6, 2008·PLoS Pathogens·Nguyen Thuy Thuong ThuongThomas R Hawn
Mar 24, 2009·Annual Review of Immunology·Andrea M Cooper
Aug 21, 2009·Nature Immunology·David G RussellFrédéric Altare
Oct 27, 2009·PLoS Pathogens·Iñaki Comas, Sebastien Gagneux
Mar 9, 2010·Anales de pediatría : publicación oficial de la Asociación Española de Pediatría (A.E.P.)·D Moreno-PérezM J Ruiz Serrano
Apr 27, 2010·Respirology : Official Journal of the Asian Pacific Society of Respirology·Keertan DhedaYing Zhang
Aug 24, 2010·The European Respiratory Journal·N Altet-GómezJ Domínguez
Sep 21, 2010·European Journal of Immunology·Katalin A Wilkinson, Robert J Wilkinson
Sep 24, 2010·Genes and Immunity·J MaertzdorfS H E Kaufmann
Oct 12, 2010·Clinical & Developmental Immunology·Stephen D LawnRobert J Wilkinson
Apr 22, 2011·European Journal of Immunology·Finlay W McNabAnne O'Garra
Mar 14, 2012·The International Journal of Tuberculosis and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·P-C ChanL-M Huang


May 14, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Simon BlankleyAnne O'Garra
May 3, 2014·The New England Journal of Medicine·Suzanne T AndersonBrian Eley
Mar 1, 2012·Expert Review of Molecular Diagnostics·M RuhwaldP Ravn
Jan 27, 2016·Human Vaccines & Immunotherapeutics·Matthew K O'Shea, Helen McShane
Sep 12, 2013·Current Opinion in Immunology·Matthew P R BerryAnne O'Garra
May 30, 2013·European Journal of Immunology·Fábio A V MarinhoSergio C Oliveira
Sep 13, 2015·Archivum Immunologiae Et Therapiae Experimentalis·Maja TravarAntonija Verhaz
Jan 29, 2016·BMC Bioinformatics·Shih-Wei LeeJulia Tzu-Ya Weng
Dec 3, 2015·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Carmen J SerranoJosé Antonio Enciso-Moreno
Oct 27, 2015·EBioMedicine·Jacqueline M AchkarEustache Paramithiotis
Oct 31, 2012·Respirology : Official Journal of the Asian Pacific Society of Respirology·C B E CheeChristoph Lange
Apr 6, 2016·Frontiers in Microbiology·Andreas DixJürgen Löffler
Feb 24, 2015·Immunological Reviews·Christopher L KarpLynda M Stuart
Oct 16, 2015·The Journal of Experimental Medicine·Jennifer L Gardiner, Christopher L Karp
Jul 21, 2015·Infectious Disease Clinics of North America·Silvia S ChiangJeffrey R Starke
May 31, 2015·Tuberculosis·Lucas Laux da CostaMaria L R Rossetti
Mar 24, 2016·BMC Medicine·Carolin T HaasMahdad Noursadeghi
Jun 15, 2018·Respirology : Official Journal of the Asian Pacific Society of Respirology·C B E CheeRobert Belknap
Sep 19, 2019·Infection and Immunity·Kathryn WrightKumudika de Silva
Jul 14, 2017·Conservation Physiology·Kristina M MillerTobi J Ming
May 8, 2021·Royal Society Open Science·Nathan B FureyScott G Hinch

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