Novel biomolecule lycopene-reduced graphene oxide-silver nanoparticle enhances apoptotic potential of trichostatin A in human ovarian cancer cells (SKOV3)

International Journal of Nanomedicine
Xi-Feng ZhangSangiliyandi Gurunathan

Abstract

Recently, there has been much interest in the field of nanomedicine to improve prevention, diagnosis, and treatment. Combination therapy seems to be most effective when two different molecules that work by different mechanisms are combined at low dose, thereby decreasing the possibility of drug resistance and occurrence of unbearable side effects. Based on this consideration, the study was designed to investigate the combination effect of reduced graphene oxide-silver nanoparticles (rGO-AgNPs) and trichostatin A (TSA) in human ovarian cancer cells (SKOV3). The rGO-AgNPs were synthesized using a biomolecule called lycopene, and the resultant product was characterized by various analytical techniques. The combination effect of rGO-Ag and TSA was investigated in SKOV3 cells using various cellular assays such as cell viability, cytotoxicity, and immunofluorescence analysis. AgNPs were uniformly distributed on the surface of graphene sheet with an average size between 10 and 50 nm. rGO-Ag and TSA were found to inhibit cell viability in a dose-dependent manner. The combination of rGO-Ag and TSA at low concentration showed a significant effect on cell viability, and increased cytotoxicity by increasing the level of malondialdehyde and...Continue Reading

Citations

Oct 24, 2018·International Journal of Molecular Sciences·Sangiliyandi GurunathanJin-Hoi Kim
Sep 20, 2020·International Journal of Molecular Sciences·Sangiliyandi GurunathanJin-Hoi Kim
Aug 30, 2020·Cancers·Lenka KoklesovaPeter Kubatka
May 6, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Manuela CurcioGiuseppe Cirillo
Nov 21, 2018·International Journal of Molecular Sciences·Sangiliyandi GurunathanKwonho Hong
Jan 17, 2019·Oxidative Medicine and Cellular Longevity·Yu-Guo YuanIl-Keun Kong
Dec 17, 2020·Frontiers in Molecular Biosciences·Gennaro SanitàAnnalisa Lamberti

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Methods Mentioned

BETA
scraping
Assay
X-ray
transmission electron microscopy
MDA
acetylation
chromosomal aberrations

Software Mentioned

GraphPad Prism

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