Novel blastospore-based transformation system for integration of phosphinothricin resistance and green fluorescence protein genes into Beauveria bassiana

Applied Microbiology and Biotechnology
Sheng-Hua Ying, Ming-Guang Feng

Abstract

A novel system was developed for efficient transformation of the fungal biocontrol agent Beauveria bassiana. Competent blastospores were prepared and stored in LiAc- and glycerol-inclusive suspension at -76 degrees C for sequential use in transformation. The system was successfully applied to integrating phosphinothricin resistance gene bar and enhanced green fluorescence protein gene egfp into B. bassiana via blastospore absorption of a plasmid vectoring bar and egfp. A frequency of 24 transformants per microgram of DNA was achieved. The blastospore-based transformation system has proven to be very convenient and would be highly potential for use in genetic manipulation of B. bassiana and other filamentous species.

References

Jan 15, 1995·FEMS Microbiology Letters·M H FungaroJ L de Azevedo
Sep 22, 1998·Nature Biotechnology·M J de GrootA G Beijersbergen
Sep 28, 1998·Molecular & General Genetics : MGG·J S BrownD W Holden
Apr 19, 2003·FEMS Yeast Research·Encarnación AndaluzGermán Larriba
Oct 8, 2003·Journal of Microbiological Methods·Lenka Pribylova, Hana Sychrova
Nov 11, 2003·Journal of Microbiological Methods·Marina KoukakiGeorge Diallinas
Jul 6, 2004·Journal of Microbiological Methods·Maria Cecília dos ReisMarcia Cristina Furlaneto
Jul 15, 2004·Advances in Applied Microbiology·Donald W Roberts, Raymond J St Leger
Jan 11, 2005·Applied and Environmental Microbiology·Weiguo FangYan Pei
Mar 16, 2005·Journal of Invertebrate Pathology·Drauzio E N RangelDonald W Roberts

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Citations

May 8, 2010·Applied Microbiology and Biotechnology·Shizhu ZhangNemat O Keyhani
Nov 27, 2010·Applied Microbiology and Biotechnology·Jinkui YangKe-Qin Zhang
Sep 28, 2012·Applied Microbiology and Biotechnology·Sheng-Hua YingNemat O Keyhani
Feb 6, 2014·World Journal of Microbiology & Biotechnology·Juanjuan LiuZhongkang Wang
Jul 20, 2014·World Journal of Microbiology & Biotechnology·Qianqian CuiZehua Zhang
Aug 16, 2015·Fungal Genetics and Biology : FG & B·Changwen ShaoZhongkang Wang
Dec 17, 2014·Applied Microbiology and Biotechnology·Almudena Ortiz-UrquizaNemat O Keyhani

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