Novel blood-based, five-gene biomarker set for the detection of colorectal cancer

Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
Mark HanChoong-Chin Liew

Abstract

We applied a unique method to identify genes expressed in whole blood that can serve as biomarkers to detect colorectal cancer (CRC). Total RNA was isolated from 211 blood samples (110 non-CRC, 101 CRC). Microarray and quantitative real-time PCR were used for biomarker screening and validation, respectively. From a set of 31 RNA samples (16 CRC, 15 controls), we selected 37 genes from analyzed microarray data that differed significantly between CRC samples and controls (P < 0.05). We tested these genes with a second set of 115 samples (58 CRC, 57 controls) using quantitative real-time PCR, validating 17 genes as differentially expressed. Five of these genes were selected for logistic regression analysis, of which two were the most up-regulated (CDA and MGC20553) and three were the most down-regulated (BANK1, BCNP1, and MS4A1) in CRC patients. Logit (P) of the five-gene panel had an area under the curve of 0.88 (95% confidence interval, 0.81-0.94). At a cutoff of logit (P) >+0.5 as disease (high risk), <-0.5 as control (low risk), and in between as an intermediate zone, the five-gene biomarker combination yielded a sensitivity of 94% (47 of 50) and a specificity of 77% (33 of 43). The intermediate zone contained 22 samples. We v...Continue Reading

Associated Clinical Trials

Citations

Nov 3, 2011·Nature Reviews. Gastroenterology & Hepatology·Nikhil PawaJohn D Norton
Apr 14, 2009·Médecine sciences : M/S·Jérôme Viguier, Martine Le Quellec Nathan
Jun 19, 2009·Journal of the National Cancer Institute·Veerle MelotteManon van Engeland
Dec 15, 2010·Infection and Immunity·Miranda S OakleySanjai Kumar
Oct 24, 2012·BMC Bioinformatics·Héctor Corrada BravoJeffrey T Leek
Jan 28, 2012·BMC Cancer·Amaia García-BilbaoMónica Betanzos
Sep 3, 2011·Journal of Clinical Bioinformatics·Soumyaroop BhattacharyaThomas J Mariani
Jan 19, 2010·Breast Cancer Research : BCR·Jørgen AarøeAnne-Lise Børresen-Dale
Oct 17, 2012·PloS One·Men Long LiongChoong Chin Liew
Oct 20, 2010·International Journal of Molecular Sciences·Takuji TanakaRikako Ishigamori
Dec 7, 2013·Molecular Diagnosis & Therapy·Karen A Heichman
Dec 17, 2008·European Journal of Endocrinology·F MorelloF Veglio
Jun 25, 2013·Pharmacology & Therapeutics·Marc TänzerMichael Quante
May 26, 2011·Clinical Colorectal Cancer·Linda J W BoschGerrit A Meijer
Aug 5, 2010·Biochemical and Biophysical Research Communications·Masao HondaUNKNOWN Hokuriku Liver Study Group
Feb 5, 2010·Alzheimer's & Dementia : the Journal of the Alzheimer's Association·Pascale Fehlbaum-BeurdeleyLaurent Bracco
Aug 3, 2013·Environmental and Molecular Mutagenesis·Cliona M McHaleMartyn T Smith
Jun 17, 2014·International Journal of Cancer. Journal International Du Cancer·Vanessa DumeauxEiliv Lund
Mar 13, 2014·Pediatrics International : Official Journal of the Japan Pediatric Society·Taketoshi YoshidaToshio Miyawaki
Dec 30, 2009·International Journal of Cancer. Journal International Du Cancer·Magnus von Knebel Doeberitz
Sep 19, 2012·Journal of Experimental & Clinical Cancer Research : CR·Adel M ZaatarChoong-Chin Liew
Oct 31, 2008·BMB Reports·Hye-Jung KimCheolju Lee
Jul 21, 2015·SpringerPlus·Dina MilowichSandrine Rorive
May 19, 2016·Lab on a Chip·Ezekiel O Adekanmbi, Soumya K Srivastava
Oct 24, 2018·American Journal of Medical Genetics. Part B, Neuropsychiatric Genetics : the Official Publication of the International Society of Psychiatric Genetics·Yuan WangChoong-Chin Liew
Oct 2, 2009·International Journal of Cancer. Journal International Du Cancer·Kenneth Wayne MarshallChoong-Chin Liew

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Genomics (Keystone)

Cancer genomics approaches employ high-throughput technologies to identify the complete catalog of somatic alterations that characterize the genome, transcriptome and epigenome of cohorts of tumor samples. Discover the latest research using such technologies in this feed.

© 2021 Meta ULC. All rights reserved