Novel cardiac precursor-like cells from human menstrual blood-derived mesenchymal cells

Stem Cells
Naoko HidaAkihiro Umezawa

Abstract

Stem cell therapy can help repair damaged heart tissue. Yet many of the suitable cells currently identified for human use are difficult to obtain and involve invasive procedures. In our search for novel stem cells with a higher cardiomyogenic potential than those available from bone marrow, we discovered that potent cardiac precursor-like cells can be harvested from human menstrual blood. This represents a new, noninvasive, and potent source of cardiac stem cell therapeutic material. We demonstrate that menstrual blood-derived mesenchymal cells (MMCs) began beating spontaneously after induction, exhibiting cardiomyocyte-specific action potentials. Cardiac troponin-I-positive cardiomyocytes accounted for 27%-32% of the MMCs in vitro. The MMCs proliferated, on average, 28 generations without affecting cardiomyogenic transdifferentiation ability, and expressed mRNA of GATA-4 before cardiomyogenic induction. Hypothesizing that the majority of cardiomyogenic cells in MMCs originated from detached uterine endometrial glands, we established monoclonal endometrial gland-derived mesenchymal cells (EMCs), 76%-97% of which transdifferentiated into cardiac cells in vitro. Both EMCs and MMCs were positive for CD29, CD105 and negative for CD...Continue Reading

References

Mar 13, 1999·The Journal of Clinical Investigation·S MakinoS Ogawa
Oct 24, 2000·The Journal of Thoracic and Cardiovascular Surgery·J S WangR C Chiu
Apr 5, 2001·Nature·D OrlicP Anversa
Jun 25, 2002·The Annals of Thoracic Surgery·Jay G ShakeBradley J Martin
May 10, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yoshihiro IijimaIssei Komuro
Jul 25, 2003·Experimental Cell Research·Satoshi GojoAkihiro Umezawa
Mar 9, 2005·Journal of Zhejiang University. Science. B·Jian-an WangBin-jian Lv
May 11, 2006·Journal of Cell Science·Lindolfo da Silva MeirellesNance Beyer Nardi
Aug 8, 2006·International Journal of Cardiology·Mai HouSheng-shou Hu
May 22, 2007·In Vitro Cellular & Developmental Biology. Animal·Masao TakeuchiHiroshi Mizusawa
Jul 24, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Robert W GraussDouwe E Atsma
Nov 17, 2007·Journal of Translational Medicine·Xiaolong MengNeil H Riordan

❮ Previous
Next ❯

Citations

Jun 11, 2011·Journal of Artificial Organs : the Official Journal of the Japanese Society for Artificial Organs·Satoshi GojoAkihiro Umezawa
Aug 1, 2012·Reviews in Endocrine & Metabolic Disorders·Caroline E GargettLouie Ye
May 22, 2010·Stem Cells and Development·Federico MosnaMauro Krampera
Jan 14, 2010·Tissue Engineering. Part a·Yi-Chun YehHsing-Wen Sung
Jun 29, 2012·Tissue Engineering. Part C, Methods·Gayathri RajaramanCaroline E Gargett
Jan 16, 2010·ILAR Journal·Valerie L Joers, Marina E Emborg
Jul 16, 2010·Molecular Human Reproduction·Caroline E Gargett, Hirotaka Masuda
Jul 22, 2009·The Journal of Clinical Investigation·Katsuhisa MatsuuraIssei Komuro
Mar 21, 2013·Journal of Translational Medicine·Leo BockeriaAmit N Patel
Aug 21, 2008·Journal of Translational Medicine·Michael P MurphyNeil H Riordan
Feb 24, 2009·Journal of Translational Medicine·Zhaohui ZhongBoris Minev
Jun 23, 2009·Journal of Translational Medicine·Tatiana JazedjeMayana Zatz
Apr 20, 2010·International Archives of Medicine·Thomas E IchimNeil H Riordan
Jun 1, 2012·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Biao ZengKai Kang
Jan 5, 2011·PloS One·Yiping FanJerry K Y Chan
Dec 1, 2012·Stem Cells Translational Medicine·Yuji HaraguchiTeruo Okano
Mar 19, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Katsuhisa MatsuuraTeruo Okano
Nov 11, 2015·Human Reproduction Update·Caroline E GargettJames A Deane
Feb 22, 2012·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·N RussoA R Genazzani
Aug 14, 2009·Expert Review of Cardiovascular Therapy·Juan C Chachques
Aug 30, 2014·International Journal of Molecular Sciences·Naoki TajiriYuji Kaneko
Apr 11, 2013·Expert Review of Cardiovascular Therapy·Yuelin ZhangHung-Fat Tse
Aug 13, 2013·Expert Opinion on Biological Therapy·Daniela UlrichCaroline E Gargett
Jan 22, 2010·Expert Opinion on Biological Therapy·Zhongmin ZouHuiqin Sun
Jul 8, 2014·Journal of Molecular and Cellular Cardiology·Noemi PavoMariann Gyöngyösi
May 27, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Katsuhisa MatsuuraTeruo Okano
Dec 6, 2014·Journal of Translational Medicine·Yongcheng LvHao Wang
Jan 30, 2016·Journal of Translational Medicine·Peng SunHao Wang
Feb 5, 2013·Journal of Tissue Engineering and Regenerative Medicine·Yuahn-Sieh HuangKuo-Hsing Ma
Jun 5, 2012·Fertility and Sterility·Caroline E Gargett, Louie Ye

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.

Cardiac Regeneration

Cardiac regeneration enables the repair of irreversibly damaged heart tissue using cutting-edge science, including stem cell and cell-free therapy. Discover the latest research on cardiac regeneration here.