Novel cell patterning using microheater-controlled thermoresponsive plasma films

Journal of Biomedical Materials Research. Part a
Xuanhong ChengBuddy D Ratner

Abstract

A novel approach is reported for cell patterning based on addressable microheaters and a poly(N-isopropyl acrylamide) (pNIPAM) themoresponsive coating. This thermoresponsive coating is created by a radio frequency NIPAM plasma and is denoted as plasma polymerized NIPAM (ppNIPAM). Films of ppNIPAM with a good retention of monomer side-chain functionality are produced using low-power continuous plasma deposition. Cell adhesion and cell detachment tests indicate that the surface switches between adhesive and nonadhesive behaviors as a function of temperature. The use of a photolithographically fabricated microheater array allows the ppNIPAM transition to occur spatially under the control of individual heaters. This localized change in the surface adhesive behavior is used to direct site-specific cell attachment. Patterned adhesion of two types of cells has been visualized on the array through fluorescent markers. Applications for diagnostic devices, cell-based sensors, tissue engineering, and cell transfection are envisioned.

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Citations

Dec 11, 2008·Biomedical Microdevices·Ryan S SincicBruce K Gale
Aug 11, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Xuanhong ChengBuddy D Ratner
Jun 29, 2012·Lab on a Chip·Edward J FeltonDaniel H Reich
Mar 29, 2007·Proceedings of the National Academy of Sciences of the United States of America·Elliot E Hui, Sangeeta N Bhatia
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Jan 8, 2010·International Journal of Molecular Sciences·Ming NiHanry Yu
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Apr 11, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Huang-Chiao HuangKaushal Rege
Feb 26, 2013·Colloids and Surfaces. B, Biointerfaces·Yoshikazu KumashiroTeruo Okano
Jul 6, 2015·Acta Biomaterialia·Tobias BechererMarie Weinhart
Aug 9, 2005·Journal of Biomedical Materials Research. Part a·Heather E CanavanDavid G Castner
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Jun 6, 2019·Annual Review of Biomedical Engineering·Buddy D Ratner
May 26, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Marta A Cooperstein, Heather E Canavan
May 31, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Abdon Pena-FranceschSalvador Borrós
Oct 23, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Lei YangJian R Lu
Nov 15, 2005·Biomacromolecules·Donald T HaynieNaveen Palath
Jul 17, 2004·Annual Review of Biomedical Engineering·Buddy D Ratner, Stephanie J Bryant

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