Novel cellulases from an extremophilic filamentous fungi Penicillium citrinum: production and characterization.

Journal of Industrial Microbiology & Biotechnology
T DuttaSanjay Ghosh

Abstract

The enzymatic hydrolysis of cellulose has potential economical and environment-friendly applications. Therefore, discovery of new extremophilic cellulases is essential to meet the requirements of industry. Penicillium citrinum (MTCC 6489) that was previously isolated from soil in our laboratory, produced alkali tolerant and thermostable cellulases. Endoglucanase and filter paper activity hydrolase (FPAse) production of P. citrinum were studied using wheat bran substrate in solid state and submerged culture. Zymogram analysis of endoglucanase revealed the presence of two isoforms differing in molecular weight. One of them was 90 kDa and other one was 38 kDa. Partially purified endoglucanase showed two different peaks at pH 5.5 and 8.0, respectively, in its pH optima curve. But FPase showed only one peak (at pH 6.5) in its pH optima curve. Cellulase of P. citrinum is thermostable in nature. The present work reports for the first time, the alkali stable cellulase from alkali tolerant fungus Penicillium citrinum. Thermostable endoglucanase from P. citrinum may have potential effectiveness as additives to laundry detergents.

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Citations

Dec 16, 2011·Applied Microbiology and Biotechnology·Ya-Shan ChengRey-Ting Guo
Jan 29, 2011·Applied Biochemistry and Biotechnology·Shanmugam JayashreeSundaram Seshadri
Jul 23, 2011·Applied Biochemistry and Biotechnology·Polyanna Nunes HerculanoAna Lúcia F Porto
Dec 23, 2008·BMC Microbiology·Sumera Afzal KhanJong-Guk Kim
Dec 6, 2012·Biotechnology Research International·Karin VegaMarcel Gutiérrez-Correa
Dec 15, 2010·Applied Biochemistry and Biotechnology·Fábio Nuno Marques Da VinhaRosalie Reed Rodrigues Coelho
May 17, 2011·Applied Biochemistry and Biotechnology·Maíra Nicolau de AlmeidaSebastião Tavares de Rezende
Jun 9, 2014·Antonie van Leeuwenhoek·Myung Soo ParkYoung Woon Lim
Jun 27, 2012·Journal of Basic Microbiology·Vidhi ChaudharyAshok Kumar Bhatnagar
Jun 3, 2009·Journal of Applied Microbiology·Y ChengY Qu
Apr 16, 2013·BioMed Research International·Marcella Novaes Franco-CiriglianoRosalie Reed Rodrigues Coelho
Oct 11, 2014·Environmental Science and Pollution Research International·Cédric TarayrePhilippe Thonart
Jul 30, 2014·Mycobiology·Myung Soo ParkYoung Woon Lim
Sep 21, 2011·Critical Reviews in Biotechnology·Anuj K ChandelSilvio Silvério da Silva
Oct 1, 2016·The Journal of Microbiology·Myung Soo ParkYoung Woon Lim
Jul 25, 2017·Current Microbiology·Prakriti Sharma Ghimire, Cheng Jin
Jan 30, 2020·Current Pharmaceutical Biotechnology·Junmei ZhouYongchang Qian

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