Novel cellulose/hydroxyapatite scaffolds for bone tissue regeneration: In vitro and in vivo study

Journal of Tissue Engineering and Regenerative Medicine
Povilas DaugelaPedro Sousa Gomes

Abstract

Cellulose scaffolds containing nano- or micro-hydroxyapatite (nHA or μHA) were prepared by the regeneration of cellulose from its acetylated derivative and the mechanical immobilization of inorganic particles, followed by freeze-drying. Microtomographic (micro-computed tomography) evaluation revealed that both scaffolds presented a highly interconnected porous structure, with a mean pore diameter of 490 ± 94 and 540 ± 132 μm for cellulose/nHA and cellulose/μHA, respectively. In vitro and in vivo characterizations of the developed scaffolds were investigated. Commercially available bone allograft was used as a control material. For the in vitro characterization, osteoblastic cell cultures were used and characterized over time to evaluate cell adhesion, metabolic activity, and functional output (alkaline phosphatase activity and osteoblastic gene expression). The results revealed greater spreading cell distribution alongside an increased number of filopodia, higher MTT values, and significantly increased expression of osteoblastic genes (Runx-2, alkaline phosphatase, and BMP-2) for cellulose/nHA, compared with cellulose/μHA and the control. The in vivo biocompatibility was evaluated in a rabbit calvarial defect model. The investi...Continue Reading

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Citations

Oct 22, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Tianyi ZhengXiaoping Yang
Aug 29, 2021·Journal of Materials Science. Materials in Medicine·João Paulo Dos Santos PradoRenata Neves Granito

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