Novel cholesterol-based cationic lipids as transfecting agents of DNA for efficient gene delivery

International Journal of Molecular Sciences
Jia JuBang-Le Zhang

Abstract

The design, synthesis and biological evaluation of the cationic lipid gene delivery vectors based on cholesterol and natural amino acids lysine or histidine are described. Cationic liposomes composed of the newly synthesized cationic lipids 1a or 1b and neutral lipid DOPE (1,2-dioleoyl-L-α-glycero-3-phosphatidyl-ethanolamine) exhibited good transfection efficiency. pEGFP-N1 plasmid DNA was transferred into 293T cells by cationic liposomes formed from cationic lipids 1a and 1b, and the transfection activity of the cationic lipids was superior (1a) or parallel (1b) to that of the commercially available 3β-[N-(N',N'-dimethylaminoethyl)-carbamoyl] cholesterol (DC-Chol) derived from the same cholesterol backbone with different head groups. Combined with the results of agarose gel electrophoresis, transfection experiments with various molar ratios of the cationic lipids and DOPE and N/P (+/-) molar charge ratios, a more effective formulation was formed, which could lead to relatively high transfection efficiency. Cationic lipid 1a represents a potential agent for the liposome used in gene delivery due to low cytotoxicity and impressive gene transfection activity.

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Citations

Jul 11, 2020·Biomimetics·Ana Maria Carmona-Ribeiro, Yunys Pérez-Betancourt
Aug 17, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Oleg V MarkovMarina A Zenkova
Sep 25, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ehexige EhexigeHuricha Baigude
Jan 23, 2021·RSC Medicinal Chemistry·Widchaya RadchatawedchakoonBoon-Ek Yingyongnarongkul
Jul 3, 2021·Pharmaceutics·Pavel A Puchkov, Michael A Maslov
Nov 4, 2021·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Bappa Maiti, Santanu Bhattacharya

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Methods Mentioned

BETA
transfection
column chromatography
dynamic light scanning
dynamic light scattering
transfections
fluorescence microscopy
flow cytometry
transfect
NMR

Software Mentioned

ELS

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