PMID: 6160469Aug 11, 1980Paper

Novel classes of mouse repeated DNAs

Nucleic Acids Research
L Manuelidis

Abstract

Mouse DNA cleaved with Eco R11 (bst NI) displays two prominent restriction bands of 1.5 and 1.7 kb in agarose gels stained with ethidium bromide. These constitute novel subsets of repeated DNA in the mouse. Sequential Hoechst 33258-CsCl gradient fractionation of mouse DNA, yielding more GC rich main band DNA, and AT rich satellite DNA, revealed that both these fragments copurified with GC rich main band DNA. They were not detected in purified satellite preparations. Together these restriction bands constituted larger than or equal to 0.2% of main band DNAs. Hybridization of 32p labelled satellite DNA to blots of Eco R11 restricted mouse DNA showed positive hybridization only to smaller satellite restriction fragments, indicating satellite DNA had little or no homology with either the 1.5 or 1.7 kb fragments. The 1.5 and 1.7 kb fragments were isolated from gels and labelled with 32p by nick translation. Using a series of restriction endonucleases each of these two fragments showed different cleavage patterns. Filter hybridization confirmed that these two fragments were distinct subsets as they did not cross hybridize with each other. They also did not hybridize to other more minor repeated non-satellite DNA bands noted in ethidi...Continue Reading

References

May 15, 1976·International Journal of Cancer. Journal International Du Cancer·L Manuelidis, E E Manuelidis
May 10, 1979·Chromosoma·L Manuelidis, E E Manuelidis
Apr 1, 1977·Analytical Biochemistry·L Manuelidis
Mar 22, 1978·Chromosoma·L Manuelidis
Jan 25, 1976·Journal of Molecular Biology·T R Cech, J E Hearst
Nov 1, 1976·Nucleic Acids Research·L Manuelidis
May 5, 1975·Journal of Molecular Biology·P A BiroP M Walker
Jul 15, 1976·Nature·H Cooke
May 5, 1975·Journal of Molecular Biology·E M Southern
Aug 22, 1975·Biochemistry·W F MarzluffR C Huang
Jun 14, 1972·Nature: New Biology·D E Comings, E Avelino
Jun 12, 1970·Science·M L Pardue, J G Gall
Apr 17, 1980·Nature·L E Orgel, F H Crick

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Citations

Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M Meunier-RotivalG Bernardi
May 25, 1982·Nucleic Acids Research·L Manuelidis, P A Biro
Aug 11, 1983·Nucleic Acids Research·T G Fanning
Mar 11, 1981·Nucleic Acids Research·N L HaigwoodM H Edgell
Apr 5, 1983·Journal of Molecular Biology·S D Brown, M Piechaczyk
Oct 25, 1983·Journal of Molecular Biology·W Gebhard, H G Zachau
Feb 1, 1986·Molecular and Cellular Biology·E D'AmbrosioA V Furano
May 25, 1982·Journal of Molecular Biology·W GebhardH G Zachau

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