Novel concentration-killing curve method for estimation of bactericidal potency of antibiotics in an in vitro dynamic model

Antimicrobial Agents and Chemotherapy
Y Q LiuP J Gao

Abstract

The bactericidal pharmacodynamics of antibiotics against Escherichia coli were analyzed by a concentration-killing curve (CKC) approach, and the novel parameters median bactericidal concentration (BC(50)) and bactericidal intensity (r) for bactericidal potency were proposed. By using the agar plate method, about 500 E. coli cells were inoculated onto Luria-Bertani plates containing a series of antibiotic concentrations, and after 24 h of incubation at 37 degrees C, all the viable colonies were enumerated. This resulted in a sigmoidal CKC that could be perfectly fitted (R(2) > 0.9) with the function N = N(0)/[1 + e(r(x - BC(50)))], where N is number of colonies surviving on each plate with an x series of concentrations of an antibiotic, and N(0) represents the meaningful inoculum size. Construction of the CKC method was based on the bactericidal effect of each antibiotic against the bacterial strain versus the concentration in two dimensions and may be a more valid, accurate, and reproducible method for estimating the bactericidal effect than the endpoint minimum bactericidal concentration (MBC) method. Mathematically, the CKC approach was point symmetrical toward its inflexion (BC(50), N(0)/2); thus, 2BC(50) could replace MBC. ...Continue Reading

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Citations

Jul 9, 2013·Journal of Biomaterials Science. Polymer Edition·Yasmine Mendes PupoJoão Carlos Gomes
Nov 17, 2010·Antimicrobial Agents and Chemotherapy·M AndraudM Laurentie
Sep 29, 2007·Science in China. Series C, Life Sciences·YuQing LiuPeiJi Gao
Nov 4, 2010·Science China. Life Sciences·HuaiQiang ZhangPeiJi Gao
Sep 2, 2009·Analytical Biochemistry·Nives SkrljMarko Dolinar
Nov 29, 2016·Scientific Reports·Iona K PatersonNick G H Taylor

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