Novel conducting polymer electrolyte biosensor based on poly(1-vinyl imidazole) and poly(acrylic acid) networks

Langmuir : the ACS Journal of Surfaces and Colloids
Ahu ArslanAyhan Bozkurt

Abstract

Biosensor construction and characterization studies of poly(acrylic acid) (PAA) and poly(1-vinyl imidazole) (PVI) complex systems have been carried out. The biosensors were prepared by mixing PAA with PVI at several stoichiometric ratios, x (molar ratio of the monomer repeat units). The enzyme, invertase, was entrapped in the PAA/PVA interpenetrating polymer networks during complexation. Modifications were made on the PAA/PVI conducting polymer electrolyte matrixes to improve the stability and performance of the polymer electrolyte-based enzyme biosensor. The maximum reaction rate (V(max)) and Michaelis-Menten constant (K(m)) were investigated for the immobilized invertase. The temperature and pH optimization, operational stability, and shelf life of the polymer electrolyte biosensor were also examined.

References

Feb 15, 2000·Journal of Biomaterials Science. Polymer Edition·S AlkanY Hepuzer

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Citations

Jul 20, 2006·Analytical and Bioanalytical Chemistry·Xinhui LouLin He
May 28, 2009·Biotechnology Advances·S M Kotwal, V Shankar
Jun 5, 2020·Dalton Transactions : an International Journal of Inorganic Chemistry·Wujie DongFuqiang Huang
Oct 27, 2020·Research : a Science Partner Journal·Wujie DongFuqiang Huang
Dec 9, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Christopher PeschelDaniel Sebastiani

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