Novel conserved group A streptococcal proteins identified by the antigenome technology as vaccine candidates for a non-M protein-based vaccine.

Infection and Immunity
Andrea FritzerAndreas Meinke

Abstract

Group A streptococci (GAS) can cause a wide variety of human infections ranging from asymptomatic colonization to life-threatening invasive diseases. Although antibiotic treatment is very effective, when left untreated, Streptococcus pyogenes infections can lead to poststreptococcal sequelae and severe disease causing significant morbidity and mortality worldwide. To aid the development of a non-M protein-based prophylactic vaccine for the prevention of group A streptococcal infections, we identified novel immunogenic proteins using genomic surface display libraries and human serum antibodies from donors exposed to or infected by S. pyogenes. Vaccine candidate antigens were further selected based on animal protection in murine lethal-sepsis models with intranasal or intravenous challenge with two different M serotype strains. The nine protective antigens identified are highly conserved; eight of them show more than 97% sequence identity in 13 published genomes as well as in approximately 50 clinical isolates tested. Since the functions of the selected vaccine candidates are largely unknown, we generated deletion mutants for three of the protective antigens and observed that deletion of the gene encoding Spy1536 drastically redu...Continue Reading

References

Jul 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·E Hanski, M Caparon
Jun 1, 1966·The Journal of Hygiene·R C Lancefield, E H Freimer
Aug 18, 1999·Proceedings of the National Academy of Sciences of the United States of America·P A LevinA D Grossman
May 19, 2000·Methods : a Companion to Methods in Enzymology·U Ringdahl, U Sjöbring
Jul 25, 2000·Clinical Microbiology Reviews·M W Cunningham
Jun 21, 2001·Proceedings of the National Academy of Sciences of the United States of America·S D ReidJ M Musser
Aug 21, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·R GonzalesM A Sande
Apr 19, 2002·The New England Journal of Medicine·Judith M MartinEllen R Wald
May 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·Hildegard EtzAndreas Meinke
Oct 2, 2002·European Journal of Immunology·Faiza RharbaouiCarlos A Guzmán
Mar 26, 2003·Infection and Immunity·Kimmo VirtanevaJames M Musser
Apr 8, 2003·The Lancet Infectious Diseases·A L BisnoC M Collins
Jun 7, 2003·Journal of Clinical Microbiology·Sebastián AlbertíUNKNOWN Spanish Surviellance Group for Respiratory Pathogens
Oct 21, 2004·International Journal of Medical Microbiology : IJMM·Bernd KreikemeyerAndreas Podbielski
Aug 10, 2005·The Journal of Infectious Diseases·Robert J EdwardsShiranee Sriskandan
Oct 29, 2005·The Lancet Infectious Diseases·Jonathan R CarapetisMartin Weber
Apr 20, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kellen C FaéLuiza Guilherme
Jul 21, 2006·Research in Microbiology·Virginie TsilibarisLaurence Van Melderen
Aug 1, 2006·Protein Science : a Publication of the Protein Society·Tatyana V RotanovaAlexander Wlodawer
Oct 3, 2006·Journal of Bacteriology·Matthew T G HoldenJulian Parkhill

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Citations

Jun 2, 2011·Proceedings of the National Academy of Sciences of the United States of America·Oretta FincoRenata Grifantini
Jun 21, 2011·Omics : a Journal of Integrative Biology·Fabio BagnoliRino Rappuoli
Jan 19, 2013·The Pediatric Infectious Disease Journal·Andrew C SteerJonathan R Carapetis
Aug 28, 2012·Journal of Bacteriology·Mohammad Shahnoor Hossain, Indranil Biswas
Feb 18, 2016·The Journal of Infection·Mark ReglinskiShiranee Sriskandan
Aug 5, 2014·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Varun K GuptaAnuradha Chakraborti
Mar 4, 2011·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Jenny Fernebro
Oct 5, 2011·Letters in Applied Microbiology·W XuC Jia
Apr 2, 2016·Vaccine·Andrew C SteerPierre R Smeesters
Apr 26, 2013·Vaccine·James B DaleAna Maria Henao-Restrepo
Feb 6, 2017·International Journal of Molecular Sciences·Ashley I HeinsonChristopher H Woelk
Feb 3, 2015·Current Protocols in Microbiology·Peter Michael Moyle
Jun 7, 2018·Human Vaccines & Immunotherapeutics·Victoria OzberkMichael F Good
Jan 30, 2015·Clinical and Vaccine Immunology : CVI·James B DaleHarry S Courtney
Apr 4, 2014·Clinical Microbiology Reviews·Mark J WalkerVictor Nizet
Nov 11, 2015·Journal of Bacteriology·Saswati BiswasIndranil Biswas
Aug 5, 2016·Clinical and Experimental Vaccine Research·Jean-Louis Excler, Jerome H Kim
Mar 19, 2011·Current Opinion in Infectious Diseases·Nicola N LynskeyShiranee Sriskandan
Mar 23, 2021·World Journal of Microbiology & Biotechnology·Abhinay SharmaAtul Kumar Johri
May 22, 2019·Microbiology Spectrum·Vincent A Fischetti

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