Novel cryptosporidium genotypes in sporadic cryptosporidiosis cases: first report of human infections with a cervine genotype

Emerging Infectious Diseases
Corinne S L OngJudith L Isaac-Renton

Abstract

In this study, we genotyped parasites from the fecal specimens of sporadic cryptosporidiosis cases in British Columbia from 1995 to 1999. Genotyping was conducted by polymerase chain amplification of the internal transcribed spacer region, a hypervariable region in the 18S rRNA gene and the Cryptosporidium oocyst wall protein gene. Subsequent analysis was by restriction fragment length polymorphism and DNA sequencing. We identified two new Cryptosporidium genotypes in humans. One of these genotypes has been found recently in deer in New York state. The other genotype has not been identified in humans or animals. These results have important implications for drinking water quality strategies, especially for communities that obtain drinking water supplies from surface sources located in forested regions with deer populations.

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Datasets Mentioned

BETA
AF087575
AF112569
AF087576
AF093490
AF087574
AF112576
AF115377
AF247535
AF112571
AF297511

Methods Mentioned

BETA
PCR
restriction profiles

Software Mentioned

TreeView
BLAST
ClustalX
SeqManII
ProRFLP

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