Novel diagnostic and clinical aspects of anti-PCNA antibodies detected by novel detection methods

Lupus
Michael MahlerMarvin J Fritzler

Abstract

Autoantibodies targeting the proliferating cell nuclear antigen have been considered as a specific biomarker for systemic lupus erythematosus, and were historically identified by indirect immunofluorescence and then confirmed by other more specific immunoassays. Our objective was to investigate the anti-PCNA immune response in various disease conditions. Unselected sera referred to a clinical diagnostic laboratory and other sera from various diseases cohorts and controls were tested for anti-PCNA antibodies by enzyme-linked immunosorbent assay (ELISA), line immunoassay (LIA) and an addressable laser bead assay (ALBIA) using full-length human proliferating cell nuclear antigen. Two out of 2500 sequential, unselected sera (0.07%) referred to a diagnostic laboratory for autoantibody analysis showed a proliferating cell nuclear antigen-like staining pattern. Good agreement was found between ELISA, ALBIA and LIA. At cut-off values resulting in 100% specificity, 52.5% (ELISA), 42.5% (ALBIA) and 35% (LIA) of samples with a proliferating cell nuclear antigen-like indirect immunofluorescence staining pattern were positive. In the indirect immunofluorescence proliferating cell nuclear antigen immunoblot (IB)-positive group, anti-PCNA ant...Continue Reading

References

Apr 2, 1987·Nature·R BravoH Macdonald-Bravo
Apr 1, 1984·The Journal of Experimental Medicine·Y TakasakiE M Tan
Mar 20, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·Y TakasakiH Hashimoto
Oct 27, 2004·Seminars in Arthritis and Rheumatism·Yaniv ShererYehuda Shoenfeld
Jul 15, 2005·Annals of the New York Academy of Sciences·J DamoiseauxJ W Cohen Tervaert
Oct 11, 2005·Joint, Bone, Spine : Revue Du Rhumatisme·Odile Beyne-RauzyFrançoise Fortenfant
Sep 18, 2007·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Rahima A BhanjiMarvin J Fritzler
Nov 13, 2008·Arthritis Research & Therapy·Michael MahlerMarvin J Fritzler

❮ Previous
Next ❯

Citations

Feb 2, 2013·Current Opinion in Rheumatology·Paul GatenbyAshwin Swaminathan
Oct 5, 2015·Joint, Bone, Spine : Revue Du Rhumatisme·Aurélien GuffroyAnne-Sophie Korganow
Mar 12, 2013·Clinical & Developmental Immunology·Makoto MiyaraMichael Mahler
Mar 3, 2012·Autoimmunity Reviews·Michael MahlerMarvin J Fritzler
Jun 13, 2015·PloS One·Kishore MalyavanthamMurali Ramanathan
Jul 29, 2018·Pediatric Rheumatology Online Journal·Ausra SnipaitieneLina Jankauskaite

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.