Novel differential linear B-cell epitopes to identify Zika and dengue virus infections in patients

Clinical & Translational Immunology
Siti Naqiah AmrunLisa F P Ng

Abstract

Recent Zika virus (ZIKV) outbreaks challenged existing laboratory diagnostic standards, especially for serology-based methods. Because of the genetic and structural similarity of ZIKV with other flaviviruses, this results in cross-reactive antibodies, which confounds serological interpretations. Plasma from Singapore ZIKV patients was screened longitudinally for antibody responses and neutralising capacities against ZIKV. Samples from healthy controls, ZIKV patients and DENV patients were further assessed using ZIKV and DENV peptides of precursor membrane (prM), envelope (E) or non-structural 1 (NS1) viral proteins in a peptide-based ELISA for epitope identification. Identified epitopes were re-validated and diagnostically evaluated using sera of patients with DENV, bacteria or unknown infections from Thailand. Long-lasting ZIKV-neutralising antibodies were elicited during ZIKV infection. Thirteen potential linear B-cell epitopes were identified, and of these, four common flavivirus, three ZIKV-specific and one DENV-specific differential epitopes had more than 50% sensitivity and specificity. Notably, ZIKV-specific peptide 26 on domain I/II of E protein (amino acid residues 271-288) presented 80% sensitivity and 85.7% specifici...Continue Reading

References

Nov 27, 2004·Journal of Virology·Wayne D Crill, Gwong-Jen J Chang
Mar 2, 2006·Transactions of the Royal Society of Tropical Medicine and Hygiene·Agus SuwandonoPatrick J Blair
Apr 21, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Zhisheng HerLisa F P Ng
Jan 29, 2014·Trends in Microbiology·Jane P MessinaSimon I Hay
Apr 8, 2015·The American Journal of Tropical Medicine and Hygiene·Lee-Ching NgChong Chee Kheong
May 8, 2015·Nature Protocols·Lawrence A KelleyMichael J E Sternberg
Jun 19, 2015·Journal of Virology·Rodolphe HamelDorothée Missé
Sep 25, 2015·Emerging Infectious Diseases·Gubio S CamposSilvia I Sardi
Mar 10, 2016·American Journal of Public Health·Maria G TeixeiraLaura C Rodrigues
Apr 19, 2016·Nature Structural & Molecular Biology·Hao SongGeorge F Gao
Jun 25, 2016·Nature·Giovanna Barba-SpaethFélix A Rey
Jun 30, 2016·Proceedings of the National Academy of Sciences of the United States of America·Lalita PriyamvadaJens Wrammert
Aug 16, 2016·The Journal of Infectious Diseases·Anna B Kawiecki, Rebecca C Christofferson
Aug 20, 2016·Nature Immunology·Stephen C Harrison
Nov 29, 2016·Journal of Clinical and Diagnostic Research : JCDR·Mohamad Adam Bujang, Tassha Hilda Adnan
Dec 8, 2016·PLoS Neglected Tropical Diseases·Sebastian FunkW John Edmunds
Dec 23, 2016·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Katja SteinhagenWolfgang Schlumberger
Jan 18, 2017·EBioMedicine·Susan J WongPei-Yong Shi
Mar 28, 2017·The Journal of Infection·Po Ying ChiaYee Sin Leo
May 22, 2017·The Lancet Infectious Diseases·UNKNOWN Singapore Zika Study Group
Jul 4, 2017·Bulletin of the World Health Organization·Hsiao-Han ChangMarc Lipsitch
Jul 19, 2017·Proceedings of the National Academy of Sciences of the United States of America·Angel BalmasedaDavide Corti
Aug 11, 2017·Journal of Clinical Microbiology·Heather L WilsonMichael Catton
Sep 29, 2017·Science Translational Medicine·Irene BoschLee Gehrke
Oct 12, 2017·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Ana Carolina Bernardes TerzianMaurício Lacerda Nogueira
Dec 28, 2017·PLoS Neglected Tropical Diseases·Li Wei AngRaymond T P Lin
Jan 3, 2018·The Journal of Infectious Diseases·Adriana GoncalvesAnnelies Wilder-Smith
Mar 3, 2018·Frontiers in Cellular and Infection Microbiology·Miguel A Martín-AcebesNereida Jiménez de Oya
Jun 1, 2018·The American Journal of Tropical Medicine and Hygiene·Jayanthi RajarethinamLee-Ching Ng

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Citations

Mar 3, 2020·Frontiers in Microbiology·André Luís Elias MoreiraClayton Luiz Borges
May 30, 2021·Virology·Utkarsh BhardwajSunit K Singh
Aug 31, 2021·Frontiers in Cellular and Infection Microbiology·Marcilio Jorge FumagalliVictor Hugo Aquino

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Datasets Mentioned

BETA
KF973479
AY099336

Methods Mentioned

BETA
ELISA
flow cytometry
PCA
PCR

Software Mentioned

Phyre
FlowJo
GraphPad Prism
R Foundation for
MultiExperiment Viewer
PyMol
GraphPad
R
Microarray Software Suite

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