Novel distance-progesterone-combined selection approach improves human sperm quality

Journal of Translational Medicine
Kun LiHe-Feng Huang

Abstract

Sperm selection is essential for the health of offspring conceived via assistive reproductive technology (ART). Various methods of sperm preparation for in vitro fertilization and intracytoplasmic sperm injection have been developed to acquire sperm with better quality and to avoid potential genetic disorders. However, current sperm processing and selection techniques bypass the natural selection that occurs during fertilization in vivo. The aim of this study was to present a novel distance-progesterone-combined selection approach with an original device based on the human female reproductive tract, and to report on its effectiveness based on sperm progressive motility, as well as chemotaxis. A novel device with long distance channels which mimicked the female human reproductive system was designed and fabricated. This ready-to-be-used device was developed using a progesterone gradient and human tube fluid media. Sperm swam for 150 min in the device under conditions of 37 °C air temperature with 5% CO2 after separation from seminal plasma via discontinuous Percoll gradient treatment. The selected sperm were assessed for normal morphology using Diff-Quik staining. A chromatin diffusion assay assessed sperm for DNA fragments and ...Continue Reading

References

Nov 21, 1995·Proceedings of the National Academy of Sciences of the United States of America·A Cohen-DayagM Eisenbach
Apr 1, 1994·Biology of Reproduction·A Cohen-DayagM Eisenbach
Aug 10, 1999·Developmental Genetics·M Eisenbach
Jan 5, 2002·Human Reproduction·G R DohleL C P Govaerts
May 23, 2002·American Journal of Human Genetics·Gerald F CoxBernhard Horsthemke
Nov 2, 2002·Proceedings of the National Academy of Sciences of the United States of America·Manabu YoshidaMasaaki Morisawa
Aug 22, 2003·Reproductive Biomedicine Online·R John AitkenDennis Sawyer
Mar 10, 2004·Human Reproduction Update·P Devroey, A Van Steirteghem
Apr 12, 2006·Nature Reviews. Molecular Cell Biology·Michael Eisenbach, Laura C Giojalas
Oct 5, 2006·Journal of Assisted Reproduction and Genetics·Zhongxiang LiHefeng Huang
Jun 21, 2007·Reproductive Biomedicine Online·R John Aitken, Geoffry N De Iuliis
Dec 14, 2007·Journal of Andrology·Tamer M SaidUwe Paasch
Jul 16, 2008·Human Reproduction·R Oren-BenaroyaM Eisenbach
Dec 10, 2009·PloS One·Maria E TevesLaura C Giojalas
Dec 6, 2011·Asian Journal of Andrology·Lara TamburrinoMonica Muratori
Jun 5, 2013·Molecular Human Reproduction·L V GaticaL C Giojalas
Dec 3, 2014·Fertility and Sterility·Leonie van den HovenAlex M M Wetzels
Apr 19, 2015·Human Reproduction Update·Charles CouttonPierre F Ray
May 23, 2015·Asian Journal of Andrology·Serafin Perez-CerezalesMichael Eisenbach
Feb 5, 2016·Biotechnology Advances·Kari L RappaWaseem Asghar
Aug 29, 2017·Best Practice & Research. Clinical Obstetrics & Gynaecology·Ziru JiangHefeng Huang

❮ Previous
Next ❯

Citations

Dec 19, 2019·Frontiers in Cell and Developmental Biology·Iván Oseguera-LópezSerafín Pérez-Cerezales
Mar 25, 2021·American Journal of Men's Health·Zhi-Da ShiYi Qiu
Jul 28, 2020·Molecular and Cellular Endocrinology·Lara TamburrinoElisabetta Baldi
Nov 7, 2021·Nature Reviews. Urology·Erica T Y LeungPhilip C N Chiu

❮ Previous
Next ❯

Methods Mentioned

BETA
chip

Software Mentioned

Diff
Quik
SPSS
PASW Statistics

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis