Novel DNA assay for cytomegalovirus detection: comparison with conventional culture and pp65 antigenemia assay.

Journal of Clinical Microbiology
N VealF Lunel

Abstract

We compared conventional cytomegalovirus (CMV) isolation, rapid viral culture, a CMV pp65 antigenemia assay, and a novel CMV DNA hybrid capture system (HCS). A total of 309 blood samples from individuals in different risk groups were assessed by at least two of the methods mentioned above. Leukocytes were recovered either after centrifugation in Leucosep tubes containing 1.080 Ficoll for pp65 assay or after simple differential lysis steps for DNA detection. HCS was based on DNA hybridization with a CMV RNA probe and its capture by antibodies to DNA-RNA hybrids. The CMV pp65 lower matrix protein was detected by fluorescence with c10-c11 monoclonal antibody in formalin-fixed leukocytes. Concordant results were observed for 92.9, 78.3, and 82.7% of the patients when comparing (i) viral culture and the pp65 antigenemia assay, (ii) viral culture and HCS, and (iii) the pp65 antigenemia assay and HCS, respectively. Discordant results were observed between a positive HCS result and negative culture and/or pp65 results. These results were associated with relatively low DNA levels (< 20 pg/10(6) cells) and positive viruria. In conclusion, the pp65 antigenemia assay is a rapid and reliable method of detecting CMV and is preferable to cult...Continue Reading

References

Jan 1, 1990·The Journal of Infectious Diseases·W M DanknerS A Spector
Dec 1, 1988·Journal of Clinical Microbiology·W van der BijT H The
Mar 1, 1986·The Journal of Infectious Diseases·J D MeyersE D Thomas
Jan 1, 1995·Journal of Clinical Microbiology·M L LandryP Wetherill

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Citations

Nov 14, 1997·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·P RautenbergD Franke
Dec 23, 2004·Transplant International : Official Journal of the European Society for Organ Transplantation·María de OñaErnesto Gómez
May 27, 2003·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Pierre AbgueguenEric Pichard
Jul 1, 1998·Clinical and Diagnostic Virology·R L Hodinka
Dec 19, 2000·International Journal of Antimicrobial Agents·C C Ginocchio
Jul 17, 1998·Clinical Microbiology Reviews·M Boeckh, G Boivin

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