Novel electrochemical sensing platform for ultrasensitive detection of cardiac troponin I based on aptamer-MoS2 nanoconjugates

Biosensors & Bioelectronics
Xiujuan QiaoJianbin Zheng

Abstract

Cardiac troponin I (cTnI) is a specific and sensitive biomarker for the early diagnosis of acute myocardial infarction and for the subsequent clinical treatments. In this work, novel electrochemical sensing platform for sensing of cTnI based on aptamer-MoS2 nanoconjugates was proposed. For comparison, core-shell Au@SiO2@Au nanoparticles were also used for sensing of cTnI. The sensing schemes and electrochemical responses of the proposed sensors were investigated by electrochemical impedance spectroscopy (EIS) in 5.0 mM K3[Fe(CN)6]/K4[Fe(CN)6] (1:1) solution containing 0.1 M KCl, respectively. Results showed that the aptamer-Au@SiO2@Au based aptasensor shows a linear rage of 10 pM-10.0 μM with the detection limits of 1.23 pM For the aptamer-MoS2 nanosheets based aptasensor, the linear range for cTnI detection was from 10 pM to 1.0 μM with a lower detection limit of 0.95 pM Meanwhile, both the sensors were successfully applied for detection of cTnI in human blood samples. The two kinds of aptsensors have been successfully used for detecting of cTnI in human blood serums. Moreover, no negligible signal changes could be observed in the presence of non-targets of CK-MB and Myo, suggesting the good potential for clinic diagnosis.

Citations

Nov 10, 2020·Analytical Methods : Advancing Methods and Applications·Cunming HuJiutong Li
Dec 10, 2020·Journal of Pharmaceutical and Biomedical Analysis·Maud SavonnetArnaud Buhot
Jan 15, 2021·Diagnostics·Ragaa Abd-Ellatief, Maha Ragaa Abd-Ellatief
Oct 28, 2019·Analytical Chemistry·Erin Marie McConnellYingfu Li
Oct 2, 2021·Analytical and Bioanalytical Chemistry·Vladyslav MishynSabine Szunerits

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