Novel Electrochemiluminescence-Sensing Platform for the Precise Analysis of Multiple Latent Tuberculosis Infection Markers

ACS Applied Materials & Interfaces
Bin ZhouPeihui Yang

Abstract

Latent tuberculosis infection (LTBI) is one of the major contributing factors for the high incidence of tuberculosis, and the low contents of LTBI markers in human serum present a great challenge for the diagnosis of LTBI. Here, we reported a novel electrochemiluminescence (ECL)-sensing platform for the precise analysis of multiple LTBI markers, interferon-gamma (IFN-γ) and interleukin (IL)-2. In this approach, self-prepared carbon quantum dots (CQDs) and luminol were integrated onto gold nanoparticles (AuNPs), which were further enriched on the surface of magnetic bead (MB) to create two solid-phase ECL nanoprobes (MB@Au@CQDs and MB@Au@luminol) for improving the detection sensitivity efficiently. Graphene oxide (GO) and AuNPs were electrodeposited onto a patterned indium tin oxide (ITO) electrode with two spatially resolved areas in sequence to form two sensitive and stable sensing areas. IFN-γ-antibody (Ab)1 and IL-2-Ab1 were separately immobilized on the two sensing areas to capture the corresponding LTBI markers, which were further recognized by IFN-γ-Ab2 and IL-2-Ab2 labeled as MB@Au@CQDs and MB@Au@luminol. The ECL intensity depended linearly on the content of IFN-γ and IL-2 in the range of 0.01-1000 pg mL-1, with a low de...Continue Reading

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Citations

Sep 26, 2019·Critical Reviews in Biotechnology·Behrouz GolichenariJavad Behravan
Sep 13, 2018·Current Medicinal Chemistry·Le Minh Tu PhanTae Jung Park
Dec 5, 2018·Chemical Reviews·Christine F MarkwalterDavid W Wright

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