Novel electrospun fibers with incorporated commensal bacteria for potential preventive treatment of the diabetic foot

Nanomedicine
Manja KurečičUroš Maver

Abstract

A novel electrospun biocompatible nanofibrous material loaded with commensal bacteria for potential preventive treatment of the diabetic foot was developed. Two biocompatible polymers (carboxymethylcellulose and polyethylene oxide) were combined with a bacterium isolate from the skin located between the toes of a healthy adult (identified using a matrix-assisted laser desorption/ionization mass spectrometry-based method as a strain of Staphylococcus epidermidis). Higher bacteria loads in the material were assured through their encapsulation in polyethylenimine. The nanofibrous material was characterized using scanning electron microscopy, zeta-potential measurements and through evaluation of cell growth and viability. nanometer formation was confirmed using scanning electron microscopy, while the zeta-potential measurements revealed successful bacteria encapsulation. Viable and sufficiently growing cells were confirmed prior and after their incorporation. The prepared materials were proven suitable to deliver viable commensal bacteria in a comparable share to the Staphylococcaceae in the foot microbiome making this approach promising for preventive diabetic foot treatment.

References

Mar 1, 1987·The Journal of Investigative Dermatology·J J LeydenG F Webster
Dec 18, 2003·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Benjamin A LipskyUNKNOWN Linezolid Diabetic Foot Infections Study Group
Feb 16, 2008·The British Journal of Dermatology·A L CogenR L Gallo
Apr 29, 2008·The Journal of Allergy and Clinical Immunology·Jürgen Schauber, Richard L Gallo
Dec 17, 2009·Nature Reviews. Microbiology·Sascha Sauer, Magdalena Kliem
May 13, 2010·The Open Microbiology Journal·Viktoria GontcharovaScot E Dowd
Jun 5, 2010·Future Microbiology·Paul D Fey, Michael E Olson
Mar 17, 2011·Nature Reviews. Microbiology·Elizabeth A Grice, Julia A Segre
Sep 28, 2006·Nanotechnology·W SalalhaE Zussman
Jul 15, 2011·Macromolecular Bioscience·Suwan N JayasingheChris J Scotton
Oct 18, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Ethan GoughAmee R Manges
Mar 26, 2014·Methods in Molecular Biology·Claudia Vuotto, Gianfranco Donelli
Jul 25, 2014·The Analyst·Elisabeth Ehler, Suwan N Jayasinghe
Jan 18, 2015·Nanomedicine : Nanotechnology, Biology, and Medicine·Wei ZhuLijie Grace Zhang
Jul 23, 2015·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M DrydenS Haider

❮ Previous
Next ❯

Citations

Jan 2, 2020·International Journal of Molecular Sciences·Brixhilda DomiJuan Antonio Tamayo-Ramos
Aug 2, 2020·AAPS PharmSciTech·Panna VassZsombor K Nagy
Mar 12, 2020·Frontiers in Bioengineering and Biotechnology·Spase Stojanov, Aleš Berlec
Jun 16, 2021·Biomaterials Science·Emily Diep, Jessica D Schiffman

❮ Previous
Next ❯

Methods Mentioned

BETA
amputation
light microscopy
scanning electron microscopy

Software Mentioned

Delsa Nano
ImageJ

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.