Novel engineered tendon-fibrocartilage-bone composite with cyclic tension for rotator cuff repair

Journal of Tissue Engineering and Regenerative Medicine
Qian LiuChunfeng Zhao

Abstract

Surgical repair of rotator cuff tears presents a significant clinical challenge with high failure rates and inferior functional outcomes. Graft augmentation improves repair outcomes; however, currently available grafting materials have limitations. Although cell-seeded decellularized tendon slices may facilitate cell infiltration, promote tendon incorporation, and preserve original mechanical strength, the unique fibrocartilage zone is yet to be successfully reestablished. In this study, we investigated the biological and mechanical properties of an engineered tendon-fibrocartilage-bone composite (TFBC) with cyclic tension (3% strain; 0.2 Hz). Decellularized TFBCs seeded with bone marrow-derived mesenchymal stem cell (BMSCs) sheets and subjected to mechanical stimulation for up to 7 days were characterised by histology, immunohistochemistry, scanning electron microscopy, mechanical testing, and transcriptional regulation. The decellularized TFBC maintained native enthesis structure and properties. Mechanically stimulated TFBC-BMSC constructs displayed increased cell migration after 7 days of culture compared with static groups. The seeded cell sheet not only integrated well with tendon scaffold but also distributed homogeneousl...Continue Reading

References

Apr 2, 1999·Science·M F PittengerD R Marshak
Feb 24, 2001·The Journal of Bone and Joint Surgery. American Volume·R H CofieldC M Rowland
Mar 11, 2003·Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association·Erik L SeverudWesley M Nottage
Sep 17, 2003·Glycoconjugate Journal·Charles C Reed, Renato V Iozzo
Feb 13, 2004·The Journal of Bone and Joint Surgery. American Volume·Leesa M GalatzKen Yamaguchi
Apr 14, 2005·The American Journal of Sports Medicine·Hilary L MalcarneyGeorge A C Murrell
Jul 19, 2005·Journal of Biomechanics·Stavros ThomopoulosGuy M Genin
Mar 8, 2006·Biomaterials·Thomas W GilbertStephen F Badylak
May 9, 2006·Journal of Shoulder and Elbow Surgery·Julie BishopEvan L Flatow
Aug 8, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Joseph YangTeruo Okano
Oct 24, 2006·Matrix Biology : Journal of the International Society for Matrix Biology·Noriaki MoriToshimitsu Uede
Aug 7, 2007·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Paul B LewisBrian J Cole
Feb 12, 2008·Clinical Orthopaedics and Related Research·David Kovacevic, Scott A Rodeo
Apr 1, 2008·Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association·James L BondStephen J Snyder
Apr 3, 2008·Tissue Engineering. Part a·Jodie E MoreauGregory H Altman
May 16, 2008·The New England Journal of Medicine·Frederick A Matsen
Sep 2, 2008·Tissue Engineering. Part a·Catherine K Kuo, Rocky S Tuan
Dec 24, 2008·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Hiromichi OmaePeter C Amadio
Jun 23, 2009·Journal of Shoulder and Elbow Surgery·Atsushi YamamotoTsutomu Kobayashi
Oct 6, 2009·Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association·Jae Chul YooKyung Sub Lim
Dec 3, 2009·Tissue Engineering. Part a·Eugene Yong-Shun SeeJames Cho Hong Goh
Jan 30, 2010·Tissue Engineering. Part a·Sepideh SaberJames Chang
Mar 31, 2010·Clinical Orthopaedics and Related Research·Emilie V CheungJohn W Sperling
May 26, 2010·Proceedings of the National Academy of Sciences of the United States of America·Yoshiaki ItoHiroshi Asahara
Sep 2, 2010·Genesis : the Journal of Genetics and Development·Tsunemitsu SoedaHaruhiko Akiyama
Mar 5, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Ho-Joong JungSavio L-Y Woo
Mar 31, 2011·Journal of Tissue Engineering and Regenerative Medicine·Hiromichi OmaeChunfeng Zhao
Jan 17, 2012·Journal of Shoulder and Elbow Surgery·Angelo Del BuonoNicola Maffulli
Feb 3, 2012·The Journal of Bone and Joint Surgery. American Volume·Alexis Chiang ColvinEvan L Flatow
Feb 10, 2012·Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association·Sung-Jae KimYong-Min Chun
Feb 11, 2012·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Ting-Wu QinChunfeng Zhao
Mar 2, 2012·Journal of Biomedical Materials Research. Part a·Liang-Ju NingTing-Wu Qin
Nov 22, 2013·The Journal of Bone and Joint Surgery. American Volume·Richard C MatherGerald Williams

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