PMID: 276865May 1, 1978

Novel enzyme from rat liver that cleaves an ADP-ribosyl histone linkage

Proceedings of the National Academy of Sciences of the United States of America
H OkayamaO Hayaishi

Abstract

A novel enzyme that splits a bond between ADP-ribose and histone was discovered and partially purified from rat liver cytosol. The 105,000 X g supernatant of rat liver homogenate was precipitated by 45% saturated ammonium sulfate and then chromatographed on a DEAE-cellulose column. The enzyme activity was eluted in a single peak at about 0.2 M NaCl and clearly separated from poly(ADP-ribose) glycohydrolase which came out at 0.13 M NaCl. In contrast to the latter enzyme, this new enzyme catalyzed the spliting of a linkage between ADP-ribose and a protein portion in mono ADP-ribosylated histone H2B but little, if any, of the glycosidic ribosyl(1"-2') ribose bonds within poly(ADP-ribose). Analysis of the reaction product by paper chromatography and Dowex 1 column chromatography indicated that the split product contained the ADP-ribose moiety but was not exactly identical with ADP-ribose. Available evidence suggested that it was either an altered ADP-ribose molecule produced by a structural rearrangement or ADP-ribose itself linked to an unidentified compound. The enzyme had a pH optimum of about 6.0 and was inhibited by 80-90% in the presence of 5 mM ADP-ribose.

References

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Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·H OkayamaO Hayaishi
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Citations

May 26, 1993·Molecular and Cellular Biochemistry·D LautierG G Poirier
Jan 1, 1980·Advances in Enzyme Regulation·T SugimuraT Utakoji
Sep 30, 1986·Biochemical and Biophysical Research Communications·Y C ChangD J Graves
Mar 5, 2010·Nature Reviews. Cancer·Michèle RouleauG G Poirier
Aug 24, 1999·The Biochemical Journal·D D'AmoursG G Poirier
Jul 19, 1984·The New England Journal of Medicine·J C WilliamsS B Conley
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J MossS J Stanley
May 14, 2008·Molecular and Cellular Biology·Stuart L RultenKeith W Caldecott
Sep 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Paul O HassaMichael O Hottiger
Jul 16, 2013·Biochemistry. Biokhimii︠a︡·G A ShilovskyS I Shram
Jan 16, 1985·Biochemical and Biophysical Research Communications·K P SmithM K Jacobson
Jan 1, 1987·Analytical Biochemistry·M R PopeP W Ludden
Jan 1, 1986·Pediatric Pathology·J C WilliamsH S Rosenberg
Sep 1, 1994·Molecular and Cellular Biochemistry·T Sugimura, M Miwa
Jun 12, 2014·Molecular Cancer Research : MCR·Matthew J Schiewer, Karen E Knudsen

Related Concepts

Cytoplasmic Matrix
Exoglycosidases
Histone H7
Liver
Nucleoside Diphosphate Sugars
Poly Adenosine Diphosphate Ribose

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