Novel erythrocyte clumps revealed by an orphan gene Newtic1 in circulating blood and regenerating limbs of the adult newt

Scientific Reports
Roman M Casco-RoblesChikafumi Chiba

Abstract

The newt, a group of urodele amphibians, has outstanding ability to repeatedly regenerate various body parts, even in the terrestrial life-stage. In this animal, when the limb is amputated, a cell mass named the blastema appears on the stump and eventually gives rise to a new functional limb. Erythrocytes (red blood cells) in most non-mammalian vertebrates, including the newt, preserve their nucleus throughout their life-span, although physiological roles of such nucleated erythrocytes, other than oxygen delivery, are not known. Here we report novel behavior of erythrocytes in the newt. We identified an orphan gene Newtic1, whose transcripts significantly increased in the blastema. Newtic1 was expressed in a subset of erythrocytes that formed a novel clump (EryC). EryC formed a complex with monocytes and was circulating throughout the body. When the limb was amputated, EryCs were newly generated in the stump and accumulated into a distal portion of the growing blastema. Our data suggested that the newt erythrocytes carried multiple secretory molecules including growth factors and matrix metalloproteases, and were capable of delivering these molecules into the blastema as a form of EryCs. This study provides insight into regulat...Continue Reading

References

Jan 1, 1966·Journal of Morphology·A M Peadon, M Singer
May 16, 2003·Vascular Pharmacology·D O Bates, S J Harper
Feb 15, 2005·Developmental Biology·Vladimir VinarskyShannon J Odelberg
Oct 19, 2006·Nature Reviews. Genetics·Alejandro Sánchez Alvarado, Panagiotis A Tsonis
Sep 27, 2007·Nature Reviews. Molecular Cell Biology·Peter ten Dijke, Helen M Arthur
Jan 12, 2010·Journal of Burn Care & Research : Official Publication of the American Burn Association·Areck A UcuzianHoward P Greisler
Apr 30, 2011·Nature Protocols·Martin Miguel Casco-RoblesChikafumi Chiba
Jul 14, 2011·Nature Communications·Goro EguchiPanagiotis A Tsonis
Sep 7, 2013·Wiley Interdisciplinary Reviews. Developmental Biology·András Simon, Elly M Tanaka
Aug 15, 2014·Scientific Reports·Md Rafiqul IslamChikafumi Chiba
Oct 30, 2014·Journal of Molecular Endocrinology·Leon A Bach
Nov 12, 2015·BioMed Research International·Katie M Giger, Theodosia A Kalfa
Mar 31, 2016·Nature Communications·Hibiki Vincent TanakaChikafumi Chiba
Sep 20, 2016·Scientific Reports·Martin Miguel Casco-RoblesChikafumi Chiba
Jan 25, 2018·Nature·Sergej NowoshilowEugene W Myers

❮ Previous
Next ❯

Citations

Oct 5, 2019·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Varun B Dwaraka, S Randal Voss
Aug 13, 2021·Translational Vision Science & Technology·Weihao ChenKatia Del Rio-Tsonis

❮ Previous
Next ❯

Datasets Mentioned

BETA
MG923550
SRP034152
PRJNA231688

Methods Mentioned

BETA
amputation
PCR

Software Mentioned

Photoshop
NIH ImageJ
TOTAL
bowtie2
CPU

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.