PMID: 2115868Aug 1, 1990Paper

Novel Escherichia coli K-12 mutants impaired in S-adenosylmethionine synthesis

Journal of Bacteriology
C SatishchandranG D Markham

Abstract

S-Adenosylmethionine (AdoMet) plays a myriad of roles in cellular metabolism. One of the many roles of AdoMet in Escherichia coli and Salmonella typhimurium is as a corepressor of genes encoding enzymes of methionine biosynthesis. To investigate the metabolic effects of large reductions in intracellular AdoMet concentrations in growing cells, we constructed and examined mutants of E. coli which are conditionally defective in AdoMet synthesis. Temperature-sensitive mutants in metK, the structural gene for the S-adenosylmethionine synthetase (AdoMet synthetase) expressed in minimal medium, were constructed by in vitro mutagenesis of a plasmid-borne copy of metK. By homologous recombination, the chromosomal copy was replaced with the mutated metK gene. Both heat- and cold-sensitive mutants were examined. At the nonpermissive temperature, two such mutants had 200-fold-reduced intracellular AdoMet levels and required either methionine or vitamin B12 for growth. In the presence of methionine or vitamin B12, the mutants grew at normal rates even though the AdoMet levels remained 0.5% of wild type. A third mutant when placed at nonpermissive temperature had less than 0.2% of the normal AdoMet level and did not grow on minimal medium ev...Continue Reading

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Citations

Apr 1, 1991·Molecular & General Genetics : MGG·D Thomas, Y Surdin-Kerjan
Nov 8, 2001·The Journal of Biological Chemistry·Rosa M RegueraDavid Ordóñez
Apr 18, 2006·Biochemistry. Biokhimii︠a︡·I V ManukhovG B Zavilgelsky
Nov 16, 2016·Journal of Industrial Microbiology & Biotechnology·Hua LiGui Yang Shi
Aug 4, 1995·The Journal of Biological Chemistry·R S Reczkowski, G D Markham
Jul 11, 1998·Journal of Bacteriology·E B NewmanR D'Ari
Jan 1, 1991·Progress in Biophysics and Molecular Biology·I G OldI Saint Girons
Jan 1, 1997·Pharmacology & Therapeutics·J M MatoM A Pajares
Aug 1, 1993·Pharmacology & Therapeutics·M Kotb, A M Geller
Sep 1, 1990·Journal of Bacteriology·C SatishchandranS M Boyle
Apr 1, 1991·Journal of Bacteriology·H Su, E B Newman
Jan 1, 2006·EcoSal Plus·Elise R Hondorp, Rowena G Matthews
Dec 31, 1997·Microbiology and Molecular Biology Reviews : MMBR·D Thomas, Y Surdin-Kerjan
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M K Berlyn
Dec 1, 1993·Microbiological Reviews·M Riley

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