Novel essential residues of Hda for interaction with DnaA in the regulatory inactivation of DnaA: unique roles for Hda AAA Box VI and VII motifs

Molecular Microbiology
Kenta Nakamura, Tsutomu Katayama

Abstract

Escherichia coli ATP-DnaA initiates chromosomal replication. For preventing extra-initiations, a complex of ADP-Hda and the DNA-loaded replicase clamp promotes DnaA-ATP hydrolysis, yielding inactive ADP-DnaA. However, the Hda-DnaA interaction mode remains unclear except that the Hda Box VII Arg finger (Arg-153) and DnaA sensor II Arg-334 within each AAA(+) domain are crucial for the DnaA-ATP hydrolysis. Here, we demonstrate that direct and functional interaction of ADP-Hda with DnaA requires the Hda residues Ser-152, Phe-118 and Asn-122 as well as Hda Arg-153 and DnaA Arg-334. Structural analyses suggest intermolecular interactions between Hda Ser-152 and DnaA Arg-334 and between Hda Phe-118 and the DnaA Walker B motif region, in addition to an intramolecular interaction between Hda Asn-122 and Arg-153. These interactions likely sustain a specific association of ADP-Hda and DnaA, promoting DnaA-ATP hydrolysis. Consistently, ATP-DnaA and ADP-DnaA interact with the ADP-Hda-DNA-clamp complex with similar affinities. Hda Phe-118 and Asn-122 are contained in the Box VI region, and their hydrophobic and electrostatic features are basically conserved in the corresponding residues of other AAA(+) proteins, suggesting a conserved role f...Continue Reading

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Citations

Jan 2, 2013·Proceedings of the National Academy of Sciences of the United States of America·Kazutoshi Kasho, Tsutomu Katayama
Sep 4, 2012·The Journal of Biological Chemistry·Shogo OzakiTsutomu Katayama
Aug 31, 2013·Nucleic Acids Research·Sundari ChodavarapuJon M Kaguni
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May 18, 2013·Environmental Microbiology·Masayuki Su'etsuguTsutomu Katayama
Aug 27, 2013·Microbiology·Carmen Fernandez-FernandezJustine Collier
May 15, 2012·Journal of Structural Biology·Shogo OzakiTsutomu Katayama
Nov 3, 2010·Current Opinion in Microbiology·Alan C Leonard, Julia E Grimwade
Nov 8, 2014·Nucleic Acids Research·Kazutoshi KashoTsutomu Katayama
Jan 12, 2017·Genes·Katie H Jameson, Anthony J Wilkinson
Dec 13, 2016·The Journal of Biological Chemistry·Kazutoshi KashoTsutomu Katayama

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