Novel evolutionary lineages of the invertebrate oxytocin/vasopressin superfamily peptides and their receptors in the common octopus (Octopus vulgaris)

The Biochemical Journal
Atsuhiro KandaHiroyuki Minakata

Abstract

The common octopus, Octopus vulgaris, is the first invertebrate species that was shown to possess two oxytocin/vasopressin (OT/VP) superfamily peptides, octopressin (OP) and cephalotocin (CT). Previously, we cloned a GPCR (G-protein-coupled receptor) specific to CT [CTR1 (CT receptor 1)]. In the present study, we have identified an additional CTR, CTR2, and a novel OP receptor, OPR. Both CTR2 and OPR include domains and motifs typical of GPCRs, and the intron- exon structures are in accord with those of OT/VP receptor genes. CTR2 and OPR expressed in Xenopus oocytes induced calcium-mediated inward chloride current in a CT- and OP-specific manner respectively. Several regions and residues, which are requisite for binding of the vertebrate OT/VP receptor family with their ligands, are highly conserved in CTRs, but not in OPR. These different sequences between CTRs and OPR, as well as the amino acid residues of OP and CT at positions 2-5, were presumed to play crucial roles in the binding selectivity to their receptors, whereas the difference in the polarity of OT/VP family peptide residues at position 8 confers OT and VP with the binding specificity in vertebrates. CTR2 mRNA was present in various peripheral tissues, and OPR mRNA...Continue Reading

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Citations

Mar 5, 2008·Proceedings of the National Academy of Sciences of the United States of America·Elisabeth StafflingerCornelis J P Grimmelikhuijzen
Feb 10, 2010·Zoological Science·Tsuyoshi KawadaHonoo Satake
Jul 25, 2014·General and Comparative Endocrinology·Frank Hauser, Cornelis J P Grimmelikhuijzen
Dec 28, 2010·General and Comparative Endocrinology·Graeme J RochNancy M Sherwood
Apr 21, 2010·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Michel Anctil
Dec 7, 2007·Frontiers in Neuroendocrinology·Frank HauserCornelis J P Grimmelikhuijzen
Jul 17, 2010·Annals of the New York Academy of Sciences·Hiroyuki Minakata
Aug 21, 2013·Experimental Physiology·Christian W Gruber
Sep 24, 2013·Worm·Isabel BeetsLiliane Schoofs
Nov 3, 2016·Developmental Neurobiology·Meghan A LockardCornelia I Bargmann
Dec 14, 2016·Scientific Reports·Zita LiutkeviciuteChristian W Gruber
Aug 7, 2018·Frontiers in Physiology·Shuichi ShigenoGraziano Fiorito
May 5, 2020·Frontiers in Endocrinology·Esther A Odekunle, Maurice R Elphick
May 19, 2021·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Jirawat SaetanPrasert Sobhon
Jul 3, 2021·International Journal of Molecular Sciences·Claudia Camerino

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