Novel expression of luteinizing hormone subunit genes in the rat testis

Endocrinology
F P ZhangI Huhtaniemi

Abstract

The two gonadotropins, LH and FSH, are thought to be synthesized and secreted solely by the anterior pituitary. We present here evidence for expression of the LH beta and common alpha-subunit (C alpha) genes in the rat testis. The LH beta and C alpha-subunit messenger RNAs (mRNAs) were detected by reverse transcriptase-polymerase chain reaction in the rat testis and pituitary with primer pairs producing 247- and 199-base pair complementary DNA (cDNA) fragments, corresponding to nucleotides 154-400 of LH beta and nucleotides 250-448 of C alpha cDNA, respectively. The specificity of the cDNA species generated was verified by Southern hybridization using nested [32P]cDNA or oligonucleotide probes, and identity with the published rat LH beta and C alpha-subunit gene structures was determined by sequencing. The mRNA bands with specific hybridization to complementary RNA (cRNA) probes corresponding to nucleotides 154-368 of the rat LH beta cDNA and nucleotides 250-448 of the rat C alpha cDNA were found in the rat pituitary and testis by Northern hybridization. The major C alpha mRNA had a size of 0.8 kilobases (kb) in the pituitary and testis. The major LH beta transcripts were 0.8 and 2.7 kb in the pituitary and testis, respectively...Continue Reading

Citations

Mar 3, 2009·Journal of Molecular Neuroscience : MN·Naomi LiticheverYitzhak Koch
Nov 14, 2014·The Prostate·Shigang XiongJacek Pinski
Oct 22, 2004·Biology of Reproduction·Kristian R von SchalburgBen F Koop
Oct 31, 2017·Balsaeng'gwa saengsig·Hee Soo Kim, Sung-Ho Lee
Oct 17, 2017·Biology of Reproduction·Anushka Jayaraman, T Rajendra Kumar
Sep 20, 2008·Genesis : the Journal of Genetics and Development·Michael A CharlesSally A Camper
Jan 28, 1999·The Journal of Biological Chemistry·A M MoilanenJ J Palvimo

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