Novel expression of luteinizing hormone/chorionic gonadotropin receptor gene in rat prostates

Molecular and Cellular Endocrinology
Y X TaoC V Rao

Abstract

The reverse transcription-polymerase chain reaction (RT-PCR) amplified an expected 255 bp luteinizing hormone/chorionic gonadotropin (LH/CG) receptor sequence from rat prostates. Northern blotting demonstrated that prostates contain 4.3, 3.3, 2.6, 1.8, 0.8 and 0.2 kb LH/CG receptor transcripts. Western immunoblotting and ligand blotting demonstrated that prostates also contain an 80 kDa receptor protein which can bind 125I-labeled hCG and this binding was inhibited by excess unlabeled hCG. In situ hybridization and immunocytochemistry revealed that while the receptors are most abundant in epithelial cells, they are scarcely found in the stroma. The ventral lobe contained more receptors than the lateral lobe and the receptors in peripheral acini of the ventral lobe are higher which progressively decreased towards central acini. In summary, prostate glands express LH/CG receptor gene. The cellular, topographical and lobular distribution of receptors suggest that LH may directly regulate prostate functions.

References

May 15, 1977·American Journal of Obstetrics and Gynecology·V Rao CHF R Carman
Feb 1, 1990·The Journal of Clinical Endocrinology and Metabolism·E ReshefJ L Luborsky
Oct 1, 1991·The Journal of Endocrinology·P Davies, C L Eaton
Jan 1, 1989·Journal of Steroid Biochemistry·S S LiaoR A Hiipakka
Dec 1, 1987·The Journal of Clinical Endocrinology and Metabolism·J S TenoverW J Bremner
Aug 1, 1987·Endocrine Reviews·G R CunhaY Sugimura
Jan 1, 1987·Methods in Enzymology·L M AngererR C Angerer
Jul 1, 1983·The Journal of Clinical Endocrinology and Metabolism·J M DavidsonJ A Catania
Jan 1, 1982·The Prostate·C J JesikC Lee
Jun 9, 1994·Nature·A J LapthornN W Isaacs

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Citations

Aug 29, 2003·The Journal of Steroid Biochemistry and Molecular Biology·Ying Zhang, Maria L Dufau
Apr 29, 1998·Annual Review of Physiology·M L Dufau
Oct 17, 2013·Hormones & Cancer·Vijayakumar GovindarajA J Rao
Nov 14, 2014·The Prostate·Shigang XiongJacek Pinski
Mar 14, 2007·Molecular and Cellular Endocrinology·K ReisingerM Zygmunt
Feb 11, 1999·The Prostate·E ReiterG Hennen

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