Novel expression pattern of cytosolic Gln synthetase in nitrogen-fixing root nodules of the actinorhizal host, Datisca glomerata

Plant Physiology
Alison M BerryK Pawlowski

Abstract

Gln synthetase (GS) is the key enzyme of primary ammonia assimilation in nitrogen-fixing root nodules of legumes and actinorhizal (Frankia-nodulated) plants. In root nodules of Datisca glomerata (Datiscaceae), transcripts hybridizing to a conserved coding region of the abundant nodule isoform, DgGS1-1, are abundant in uninfected nodule cortical tissue, but expression was not detectable in the infected zone or in the nodule meristem. Similarly, the GS holoprotein is immunolocalized exclusively to the uninfected nodule tissue. Phylogenetic analysis of the full-length cDNA of DgGS1-1 indicates affinities with cytosolic GS genes from legumes, the actinorhizal species Alnus glutinosa, and nonnodulating species, Vitis vinifera and Hevea brasilensis. The D. glomerata nodule GS expression pattern is a new variant among reported root nodule symbioses and may reflect an unusual nitrogen transfer pathway from the Frankia nodule microsymbiont to the plant infected tissue, coupled to a distinctive nitrogen cycle in the uninfected cortical tissue. Arg, Gln, and Glu are the major amino acids present in D. glomerata nodules, but Arg was not detected at high levels in leaves or roots. Arg as a major nodule nitrogen storage form is not found in ...Continue Reading

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Citations

Aug 19, 2011·Molecular Plant-microbe Interactions : MPMI·David R BensonJuliana E Mastronunzio
May 9, 2009·The New Phytologist·Stéphanie M Bernard, Dimah Z Habash
Jan 10, 2020·Molecular Plant-microbe Interactions : MPMI·Anne-Emmanuelle HayHasna Boubakri
Sep 1, 2011·Functional Plant Biology : FPB·Katharina PawlowskiAlison M Berry
Sep 1, 2011·Functional Plant Biology : FPB·Alison M BerryKatharina Pawlowski

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