Novel Fabrication of MicroRNA Nanoparticle-Coated Coronary Stent for Prevention of Post-Angioplasty Restenosis

Korean Circulation Journal
Hui-Lian CheMyung-Ho Jeong

Abstract

MicroRNA 145 is known to be responsible for cellular proliferation, and its enhanced expression reportedly inhibits the retardation of vascular smooth muscle cell growth specifically. In this study, we developed a microRNA 145 nanoparticle immobilized, hyaluronic acid (HA)-coated stent. For the gene therapy, we used disulfide cross-linked low molecular polyethylenimine as the carrier. The microRNA 145 was labeled with YOYO-1 and the fluorescent microscopy images were obtained. The release of microRNA 145 from the stent was measured with an ultra violet spectrophotometer. The downstream targeting of the c-Myc protein and green fluorescent protein was determined by Western blotting. Finally, we deployed microRNA 145/ssPEI nanoparticles immobilized on HA-coated stents in the balloon-injured external iliac artery in a rabbit restenosis model. Cellular viability of the nanoparticle-immobilized surface tested using A10 vascular smooth muscle cells showed that MSN exhibited negligible cytotoxicity. In addition, microRNA 145 and downstream signaling proteins were identified by western blots with smooth muscle cell (SMC) lysates from the transfected A10 cell, as the molecular mechanism for decreased SMC proliferation that results in the...Continue Reading

References

Jun 7, 2002·The New England Journal of Medicine·Marie-Claude MoriceUNKNOWN RAVEL Study Group. Randomized Study with the Sirolimus-Coated Bx Velocity Balloon-Expandable Stent in the Treatment of Patie
Nov 20, 2002·Circulation·Mohan N Babapulle, Mark J Eisenberg
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Jan 29, 2011·International Journal of Nanomedicine·Dunwan ZhuCunxian Song

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Citations

Apr 4, 2017·The Journal of Gene Medicine·Akshaya KrishnagopalDwaipayan Sen
Feb 12, 2017·Chonnam Medical Journal·Kamali Manickavasagam LekshmiIn-Kyu Park
Dec 4, 2016·Clinical Chemistry and Laboratory Medicine : CCLM·Christian SchulteTanja Zeller
Apr 24, 2017·Polymers·Ansuja Pulickal MathewIn-Kyu Park

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Methods Mentioned

BETA
percutaneous
coronary artery bypass
transfection
scanning electron microscopy
protein assay

Software Mentioned

SPSS

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