Novel fibrin-fibronectin matrix accelerates mice skin wound healing

Bioactive Materials
C. P. JaraEliana P Araújo

Abstract

Plasma fibrinogen (F1) and fibronectin (pFN) polymerize to form a fibrin clot that is both a hemostatic and provisional matrix for wound healing. About 90% of plasma F1 has a homodimeric pair of γ chains (γγF1), and 10% has a heterodimeric pair of γ and more acidic γ' chains (γγ'F1). We have synthesized a novel fibrin matrix exclusively from a 1:1 (molar ratio) complex of γγ'F1 and pFN in the presence of highly active thrombin and recombinant Factor XIII (rFXIIIa). In this matrix, the fibrin nanofibers were decorated with pFN nanoclusters (termed γγ'F1:pFN fibrin). In contrast, fibrin made from 1:1 mixture of γγF1 and pFN formed a sporadic distribution of "pFN droplets" (termed γγF1+pFN fibrin). The γγ'F1:pFN fibrin enhanced the adhesion of primary human umbilical vein endothelium cells (HUVECs) relative to the γγF1+FN fibrin. Three dimensional (3D) culturing showed that the γγ'F1:pFN complex fibrin matrix enhanced the proliferation of both HUVECs and primary human fibroblasts. HUVECs in the 3D γγ'F1:pFN fibrin exhibited a starkly enhanced vascular morphogenesis while an apoptotic growth profile was observed in the γγF1+pFN fibrin. Relative to γγF1+pFN fibrin, mouse dermal wounds that were sealed by γγ'F1:pFN fibrin exhibited a...Continue Reading

References

Jun 1, 1978·Archivum Histologicum Japonicum = Nihon Soshikigaku Kiroku·L C JunqueiraR Brentani
Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·D A Cheresh
Jun 1, 1986·The Journal of Cell Biology·P KnoxC S Rimmer
Dec 20, 1985·Science·J W WeiselC Cohen
Jun 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·R Chen, R F Doolittle
Jul 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·P A McKeeR L Hill
Jul 1, 1966·Journal of Clinical Pathology·C M Ogston, D Ogston
Nov 1, 1982·The Journal of Cell Biology·R O Hynes, K M Yamada
Jun 27, 1983·Annals of the New York Academy of Sciences·D F Mosher, R B Johnson
Jan 1, 1984·Annual Review of Medicine·D F Mosher
Nov 1, 1995·The Journal of Cell Biology·S MiyamotoK M Yamada
May 18, 1999·The Journal of Biological Chemistry·A SahniC W Francis
Sep 2, 1999·The New England Journal of Medicine·A J Singer, R A Clark
May 12, 2000·Nature Cell Biology·D J SiegD D Schlaepfer
Jan 9, 2001·The Journal of Investigative Dermatology. Symposium Proceedings·M G TonnesenR A Clark
Jul 20, 2001·Annals of the New York Academy of Sciences·R A Clark
Apr 8, 2004·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Marco ArnoldJoachim P Spatz
Dec 19, 2007·Journal of Biomedical Materials Research. Part a·John H Slater, Wolfgang Frey
Mar 27, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Gregory S Schultz, Annette Wysocki
Nov 1, 2001·Aesthetic Surgery Journal·J B EbyD A Kulber
Aug 8, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Jon E MogfordThomas A Mustoe
Aug 19, 2009·Blood·Shirley Uitte de WilligeRobert A S Ariëns
Oct 13, 2009·World Journal of Surgery·William D Spotnitz
Oct 24, 2009·The American Journal of Pathology·Rita MirzaTimothy J Koh
May 19, 2010·Developmental Biology·Sarah CalveHans-Georg Simon
Aug 20, 2010·Science Translational Medicine·Timothy A PetrieAndrés J García
Sep 3, 2010·The Journal of Investigative Dermatology·Fubao LinRichard A F Clark
Feb 18, 2011·The Journal of Investigative Dermatology·Saher HamedLuc Teot
Aug 17, 2011·Biophysical Journal·Guillaume Le SauxKatharina Gaus

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Methods Mentioned

BETA
phase-contrast
confocal microscopy
biopsy
PCR

Software Mentioned

ImageJ
GraphPad Prism
Adobe Photoshop
Image Pro Plus
Photomerge
Labinsane App
Leica Application Suite

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