Novel flow cytometric approach for the detection of adipocyte subpopulations during adipogenesis.

Journal of Lipid Research
Chrisna DurandtMichael S Pepper

Abstract

The ability of mesenchymal stromal cells (MSCs) to differentiate into adipocytes provides a cellular model of human origin to study adipogenesis in vitro. One of the major challenges in studying adipogenesis is the lack of tools to identify and monitor the differentiation of various subpopulations within the heterogeneous pool of MSCs. Cluster of differentiation (CD)36 plays an important role in the formation of intracellular lipid droplets, a key characteristic of adipocyte differentiation/maturation. The objective of this study was to develop a reproducible quantitative method to study adipocyte differentiation by comparing two lipophilic dyes [Nile Red (NR) and Bodipy 493/503] in combination with CD36 surface marker staining. We identified a subpopulation of adipose-derived stromal cells that express CD36 at intermediate/high levels and show that combining CD36 cell surface staining with neutral lipid-specific staining allows us to monitor differentiation of adipose-derived stromal cells that express CD36(intermediate/high)during adipocyte differentiation in vitro. The gradual increase of CD36(intermediate/high/)NR(positive)cells during the 21 day adipogenesis induction period correlated with upregulation of adipogenesis-ass...Continue Reading

References

Mar 1, 1992·Experimental Cell Research·M J Smyth, W Wharton
Mar 1, 1985·The Journal of Cell Biology·P GreenspanS D Fowler
Aug 1, 1985·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S D Fowler, P Greenspan
Dec 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·R E ScottJ H Kersey
May 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·R E BarnettR E Scott
Aug 19, 2000·The Journal of Biological Chemistry·D L BrasaemleC Londos
Feb 5, 2004·Nature Medicine·Michael Lehrke, Mitchell A Lazar
Nov 25, 2004·American Journal of Physiology. Endocrinology and Metabolism·Bohan WangPaul Trayhurn
May 4, 2005·The Journal of Allergy and Clinical Immunology·Giamila Fantuzzi
Jul 21, 2005·Histochemistry and Cell Biology·Franck FestyRégis Roche
Nov 29, 2005·Best Practice & Research. Clinical Endocrinology & Metabolism·Cristiana E Juge-AubryChristoph A Meier
Jun 8, 2006·Obesity Surgery·Rosário MonteiroIsabel Azevedo
Jul 4, 2006·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Carl-Magnus BäckesjöLars-Arne Haldosén
Dec 2, 2006·Nature Reviews. Molecular Cell Biology·Evan D Rosen, Ormond A MacDougald
Aug 8, 2007·Trends in Immunology·A SchäfflerB Salzberger
Feb 26, 2008·Histochemistry and Cell Biology·Giacomo DiazAngela Maria Falchi
Jul 3, 2008·Methods : a Companion to Methods in Enzymology·Bruce A BunnellCynthia Ripoll
Aug 19, 2008·Biochimica Et Biophysica Acta·Samantha MurphyRobert G Parton
Sep 5, 2008·Current Opinion in Lipidology·Sven-Olof OlofssonJan Borén
Sep 27, 2008·Stem Cells·Susan Rainey DaherKeith L March
Dec 5, 2008·Digestive Diseases and Sciences·Susan E WozniakEldo E Frezza
Feb 3, 2009·Trends in Endocrinology and Metabolism : TEM·Xiong Su, Nada A Abumrad
Aug 18, 2009·Molecular and Cellular Endocrinology·E Maury, S M Brichard
Nov 26, 2009·Plastic and Reconstructive Surgery·Hitomi EtoKotaro Yoshimura
May 4, 2010·Trends in Immunology·Andreas Schäffler, Jürgen Schölmerich
May 22, 2010·Stem Cells and Development·Federico MosnaMauro Krampera
Jun 1, 2010·Journal of Cellular Biochemistry·Andrea ArmaniMassimiliano Caprio
Nov 10, 2010·Expert Opinion on Biological Therapy·Timothy J MyersAnna Spagnoli
Feb 4, 2011·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Kristina SchaedlichAnne Navarrete Santos
Apr 19, 2011·Trends in Endocrinology and Metabolism : TEM·Weihua FeiHongyuan Yang
Jul 20, 2011·Clinical Endocrinology·Andrew LansdownAled Rees
Jul 26, 2011·BioTechniques·Max V RanallThomas J Gonda
Aug 24, 2011·Journal of Electron Microscopy·Michitaka SuzukiToyoshi Fujimoto
Oct 1, 2011·Journal of Biomedicine & Biotechnology·Espen E SpangenburgRichard M Lovering
Apr 3, 2012·Annual Review of Biochemistry·Qi Qun Tang, M Daniel Lane
Apr 18, 2012·Biochimica Et Biophysica Acta·Valerie ChristiaensIlse Scroyen

❮ Previous
Next ❯

Citations

May 8, 2019·Tissue Engineering. Part C, Methods·Carla Dessels, Michael Sean Pepper
Feb 26, 2020·International Journal of Molecular Sciences·Qiuyue PengVladimir Zachar
May 27, 2017·Molecular Genetics & Genomic Medicine·Melvin Anyasi Ambele, Michael Sean Pepper
Jun 13, 2020·International Journal of Molecular Sciences·Carina da SilvaMichael S Pepper
Feb 23, 2020·Stem Cells International·Omair A MohiuddinJeffrey M Gimble
Apr 30, 2017·Adipocyte·Badwi B BoumelhemStuart T Fraser
Jul 23, 2019·EBioMedicine·Carina MiggitschBeatrix Grubeck-Loebenstein

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
fluorescence microscopy

Software Mentioned

GraphPad PRISM
INSTAT
Kaluza flow cytometry
GraphPad
AxioVision
Image J

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

© 2021 Meta ULC. All rights reserved