Novel form of p21(WAF1/CIP1/SDI1) protein in phorbol ester-induced G2/M arrest.

The Journal of Biological Chemistry
W W TchouK M Tchou-Wong

Abstract

Cell cycle progression requires activation of different cyclin-dependent kinases (CDKs) which are positively regulated by cyclins and negatively regulated by CDK inhibitors. Growth inhibition of the Calu-1 lung carcinoma cells induced with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA), a potent activator of protein kinase C, is associated with G2/M arrest and induction of expression of a novel, faster-migrating form of p21(WAF1/CIP1/SDI1) (p21) protein, an inhibitor of cyclin-dependent kinases. This faster-migrating p21 protein was also expressed in TPA-treated A549 lung carcinoma cells which also exhibited G2/M arrest but not in TPA-treated U937 leukemia cells, which only expressed a slower-migrating form of p21 protein. However, reverse transcriptase-polymerase chain reaction and Southern analysis demonstrated no evidence of novel splice in TPA-treated Calu-1 cells. On the other hand, immunoblotting analysis demonstrated that the faster-migrating p21 protein could be detected only by peptide antibody directed against the N terminus but not the C terminus, suggestive of truncation of the latter or protein modification that results in the loss of the C-terminal epitope. Correlation of G2/M arrest with expression ...Continue Reading

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Citations

Apr 16, 1998·Molecular Carcinogenesis·H AokiT Sakaki
Aug 26, 1998·Clinical Immunology and Immunopathology·I OkayasuM Fujiwara
May 4, 2005·Breast Cancer Research and Treatment·Shaojin YouWilliam J M Hrushesky
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Mar 23, 2019·Scientific Reports·Jian YangXin Li
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Oct 6, 2005·European Journal of Pharmacology·Wen-Ying ChenYang-Chang Wu

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