Novel frequenin-modulated Ca2+-signaling membrane guanylate cyclase (ROS-GC) transduction pathway in bovine hippocampus

Molecular and Cellular Biochemistry
Ewa Fik-RymarkiewiczRameshwar K Sharma

Abstract

Frequenin is a member of the neuronal Ca(2+) sensor protein family, implicated in being the modulator of the neurotransmitter release, potassium channels, phosphatidylinositol signaling pathway and the Ca(2+)-dependent exocytosis of dense-core granules in the PC12 cells. Frequenin exhibits these biological activities through its Ca(2+) myristoyl switch, yet the switch is functionally inactive. These structural and functional traits of frequenin have been derived through the use of recombinant frequenin. In the present study, frequenin (BovFrq) native to the bovine hippocampus has been purified, sequenced for its 9 internal fragments, cloned, and studied. The findings show that structure of the BovFrq is identical to its form present in chicken, rat, mouse and human, indicating its evolutionary conservation. Its Ca(2+) myristoyl switch is active in the hippocampus. And, BovFrq physically interacts and turns on yet undisclosed ONE-GC-like ROS-GC membrane guanylate cyclase transduction machinery in the hippocampal neurons. This makes BovFrq a new Ca(2+)-sensor modulator of a novel ROS-GC transduction machinery. The study demonstrates the presence and mechanistic features of this cyclic GMP signaling pathway in the hippocampal neur...Continue Reading

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Citations

Jan 24, 2009·Molecular and Cellular Biochemistry·Anuradha KrishnanRameshwar K Sharma
Dec 3, 2009·Molecular and Cellular Biochemistry·Rameshwar K Sharma, Teresa Duda
Dec 4, 2009·Molecular and Cellular Biochemistry·Rameshwar K Sharma
Oct 4, 2016·Frontiers in Molecular Neuroscience·Rameshwar K SharmaClint L Makino
May 31, 2007·Pflügers Archiv : European journal of physiology·Donald W Hilgemann
Nov 26, 2009·Molecular and Cellular Biochemistry·Rameshwar K Sharma, Teresa Duda
Mar 8, 2012·Molecular Neurobiology·Jeffrey S DasonAlberto Ferrús
May 27, 2008·Biochemistry·Venkateswar VenkataramanRameshwar K Sharma

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