Novel function of tenascin-C, a matrix protein relevant to atherosclerosis, in platelet recruitment and activation under flow

Arteriosclerosis, Thrombosis, and Vascular Biology
Mathieu SchaffPierre H Mangin

Abstract

The identification of platelet-reactive proteins exclusively present in atherosclerotic plaques could provide interesting targets for effective and safe antithrombotic strategies. In this context, we explored platelet adhesion and activation to tenascin-C (TN-C), a matrix protein preferentially found within atheroma. We show that platelets efficiently adhere to TN-C under both static and flow conditions. Videomicroscopy revealed a unique behavior under flow, with platelets exhibiting stationary adhesion to TN-C; in contrast, platelets rolled over von Willebrand factor and detached from fibrinogen. Platelet interaction with TN-C was predominantly supported by integrin α(2)β(1) under static conditions, whereas under high shear, it was dependent on both the α(2)β(1) integrin and the glycoprotein Ib-IX complex. Integrin α(IIb)β(3) appeared to play a secondary role but only at low shear rates. The glycoprotein Ib-IX-dependent interaction was indirect, relying on von Willebrand factor, and increased as a function of wall shear rate. Von Willebrand factor bound directly to TN-C, as shown by ELISA and coimmunoprecipitation, suggesting that it acts as a bridge between TN-C and platelets. The adhesion of platelets to TN-C triggered their...Continue Reading

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Citations

Aug 6, 2011·Cellular and Molecular Life Sciences : CMLS·Kim S MidwoodGertraud Orend
May 7, 2013·Annual Review of Biomedical Engineering·Thomas V ColaceScott L Diamond
Jun 30, 2011·Cardiovascular Research·Jonathan GolledgeSimon Koblar
Aug 21, 2015·Hämostaseologie·E Mammadova-BachC Gachet
Apr 12, 2011·Thrombosis and Haemostasis·Béatrice Hechler, Christian Gachet
Jun 30, 2012·Journal of Signal Transduction·Gianni Francesco Guidetti, Mauro Torti
Feb 16, 2018·Biological Reviews of the Cambridge Philosophical Society·Bart NieuwenhuisJames W Fawcett
May 18, 2018·Journal of Atherosclerosis and Thrombosis·Wen GaoKun Xie
Sep 1, 2017·Journal of Cellular and Molecular Medicine·Zhiyang WangXin Xie
Sep 23, 2018·Journal of Neuroscience Research·Hidenori SuzukiMasato Shiba
Nov 6, 2015·Frontiers in Oncology·Freja A VenningJanine T Erler
Jan 29, 2016·Biologie aujourd'hui·Mathieu SchaffPierre Henri Mangin
Aug 8, 2015·Thrombosis and Haemostasis·Eric MaurerPierre H Mangin
Jul 20, 2017·Scientific Reports·Bamaprasad DuttaSiu Kwan Sze
Oct 10, 2015·The International Journal of Artificial Organs·Gulsum OzkanAdnan Yavuz

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