Novel genetic context of multiple bla OXA-58 genes in Acinetobacter genospecies 3

The Journal of Antimicrobial Chemotherapy
Benjamin A EvansSebastian G B Amyes

Abstract

The detection in Acinetobacter genospecies 3 isolates of OXA-type carbapenemases, resulting in reduced susceptibility to carbapenem antibiotics, is increasingly reported. We identified an Acinetobacter genospecies 3 isolate carrying the gene for OXA-58 and aimed to resolve the genetic environment surrounding the bla(OXA-58) gene. Species identification was confirmed by 16S-23S rRNA restriction analysis. MICs of imipenem, meropenem and ertapenem were determined, and the isolate was screened by PCR for bla(OXA-23-like), bla(OXA-40-like), bla(OXA-51-like) and bla(OXA-58-like) genes. The sequence surrounding bla(OXA-58) was determined through amplification by inverse PCR and genome walking followed by sequencing. Genetic localization was investigated by Southern blotting. Isolate A164 was confirmed as belonging to Acinetobacter genospecies 3 and had reduced susceptibility to the carbapenems. The isolate was found to encode two bla(OXA-58) genes that may have been duplicated by the insertion sequence ISAba125, two copies of which were inserted into ISAba3 elements. The bla(OXA-58) genes appear to be plasmid borne. This is the first report of beta-lactamase duplication in Acinetobacter genospecies 3 and of gene duplication mediated b...Continue Reading

References

Dec 25, 2002·Antimicrobial Agents and Chemotherapy·Claire HéritierPatrice Nordmann
Mar 26, 2003·Antimicrobial Agents and Chemotherapy·Elizabeth T S HouangA F B Cheng
Mar 28, 2006·International Journal of Antimicrobial Agents·Neil WoodfordDavid M Livermore
Aug 18, 2006·Gene·Haitao Guo, Jin Xiong
Feb 8, 2007·The Journal of Antimicrobial Chemotherapy·Tse Hsien KohYi Zhao
Apr 18, 2007·Antimicrobial Agents and Chemotherapy·Alessia BertiniAlessandra Carattoli
Dec 21, 2007·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·K J TownerARPAC Steering Group
Jan 15, 2008·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Benjamin A EvansSebastian G B Amyes

Citations

Jun 2, 2011·The Journal of Antimicrobial Chemotherapy·Laurent PoirelPatrice Nordmann
Jun 23, 2011·The Journal of Antimicrobial Chemotherapy·Yvonne PfeiferPaul G Higgins
Aug 24, 2011·Antimicrobial Agents and Chemotherapy·Laurent PoirelPatrice Nordmann
Jul 7, 2011·Antimicrobial Agents and Chemotherapy·Mar SoléJordi Vila
Jul 12, 2013·Future Microbiology·Kathryn T ElliottEllen L Neidle
Feb 11, 2014·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M AlyH H Balkhy
Oct 20, 2011·Critical Reviews in Microbiology·Wei-Hua Zhao, Zhi-Qing Hu
Aug 17, 2016·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M M AlyH H Balkhy
Feb 26, 2014·Antimicrobial Agents and Chemotherapy·Esther ZanderPaul G Higgins
Apr 4, 2014·Clinical Microbiology Reviews·Benjamin A Evans, Sebastian G B Amyes
Sep 28, 2016·Antimicrobial Agents and Chemotherapy·Séverine BontronLaurent Poirel
Jun 4, 2014·Antimicrobial Agents and Chemotherapy·Rodrigo CayôLuis Martínez-Martínez
Oct 13, 2018·Journal of Medical Microbiology·Uthaibhorn Singkham-In, Tanittha Chatsuwan
Mar 11, 2015·Scientific Reports·Shuru ZhouXuemin Guo
Jan 1, 2014·Antibiotics·Nuno T Antunes, Jed F Fisher
Jan 1, 2021·The Journal of Antimicrobial Chemotherapy·Eun-Jeong Yoon, Seok Hoon Jeong
Jan 29, 2021·Microorganisms·Peechanika ChopjittAnusak Kerdsin

Related Concepts

Merrem
Invanoz
Beta-lactamase OXA-58, Acinetobacter baumannii
Mima
Acinetobacter Infections
Antibiotics
Beta-Lactamase
DNA Transposons
DNA, Bacterial
Genes, Bacterial

Related Feeds

Carbapenems (ASM)

Carbapenems are members of the beta lactam class of antibiotics and are used for the treatment of severe or high-risk bacterial infections. Discover the latest research on carbapenems here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Carbapenems

Carbapenems are members of the beta lactam class of antibiotics and are used for the treatment of severe or high-risk bacterial infections. Discover the latest research on carbapenems here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Acinetobacter Infections

Acinetobacter infections have become common in hospitalized patients, especially in the intensive care unit setting and are difficult to treat due to their propensity to develop antimicrobial drug resistance. Discover the latest research on Acinetobacter Infections here.