Novel gold nanoparticle trimer reporter probe combined with dry-reagent cotton thread immunoassay device for rapid human ferritin test

Analytica Chimica Acta
Xun MaoTingting Song

Abstract

We reported here for the first time on the use of cotton thread combined with novel gold nanoparticle trimer reporter probe for low-cost, sensitive and rapid detection of a lung cancer related biomarker, human ferritin. A model system comprising ferritin as an analyte and a pair of monoclonal antibodies was used to demonstrate the proof-of-concept on the dry-reagent natural cotton thread immunoassay device. Results indicated that the using of novel gold nanoparticle trimer reporter probe greatly improved the sensitivity comparing with traditional gold nanoparticle reporter probe on the cotton thread immunoassay device. The assay avoids multiple incubation and washing steps performed in most conventional protein analyses. Although qualitative tests are realized by observing the color change of the test zone, quantitative data are obtained by recording the optical responses of the test zone with a commercial scanner and corresponding analysis software. Under optimal conditions, the cotton thread immunoassay device was capable of measuring 10 ng/mL human ferritin under room temperature which is sensitive enough for clinical diagnosis. Moreover, the sample solution employed in the assays is just 8 μL, which is much less than tradit...Continue Reading

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Citations

Nov 24, 2016·Diagnostics·Mílton CordeiroPedro Viana Baptista
Mar 13, 2018·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Alex J SpositoIndira Hewlett
Apr 10, 2020·Royal Society Open Science·Jiahong SongLongfei Cai
Jun 1, 2021·ACS Omega·Anusha PrabhuNaresh Kumar Mani
Jan 15, 2019·ACS Combinatorial Science·Syamak FarajikhahGordon Wallace
Oct 15, 2021·Analytical Methods : Advancing Methods and Applications·Deonir AgustiniDosil Pereira de Jesus

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