Novel group V phospholipase A2 involved in arachidonic acid mobilization in murine P388D1 macrophages.

The Journal of Biological Chemistry
M A BalboaE A Dennis

Abstract

Four related genes encode four different secretory phospholipase A2 (sPLA2) enzymes in mammals, namely the well described Group I and IIA enzymes and the more recently described Groups IIC and V. A large body of research has putatively demonstrated that the Group IIA sPLA2 is involved in diverse pathologic processes, such as rheumatoid arthritis, septic shock, intestinal neoplasia, and epidermal hyperplasia, as well as in cellular signaling by regulating the formation of arachidonate-derived lipid messengers. However, we demonstrate herein the involvement of another sPLA2, i.e. the Group V sPLA2, in arachidonic acid release and prostaglandin production in the mouse macrophage-like cell line P388D1. Abundant message for Group V sPLA2 was detected in both resting and activated cells. In contrast, Group IIA sPLA2 message was undetectable as analyzed by Northern blot and reverse transcriptase-polymerase chain reaction. Moreover, blockage of Group V sPLA2 gene expression by antisense RNA oligonucleotides resulted in inhibition of prostaglandin E2 production as well as reduction of the amount of sPLA2 protein at the cellular surface. Collectively, these results uncover Group V sPLA2 as a novel effector involved in arachidonic acid-me...Continue Reading

References

Feb 1, 1995·The Journal of Clinical Investigation·S S HarwigR I Lehrer
Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·J BalsindeE A Dennis
Feb 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R J Mayer, L A Marshall
Mar 22, 1996·The Journal of Biological Chemistry·J Balsinde, E A Dennis

❮ Previous
Next ❯

Citations

Jul 27, 1999·Lipids·B ChaminadeH Chap
Aug 7, 1999·Clinical Reviews in Allergy & Immunology·M C Seeds, D A Bass
Nov 5, 2008·Archives of Pharmacal Research·S J LeeH P Kim
Oct 15, 2008·Cardiovascular Drugs and Therapy·Boris B Boyanovsky, Nancy R Webb
Oct 27, 2009·Neuromolecular Medicine·Grace Y SunAgnes Simonyi
Oct 10, 1998·Biochimica Et Biophysica Acta·Y Chen, E A Dennis
Jun 1, 2000·Advances in Enzyme Regulation·M A GijónC C Leslie
Nov 16, 2002·Prostaglandins & Other Lipid Mediators·Ichiro Kudo, Makoto Murakami
Feb 19, 2000·Prostaglandins & Other Lipid Mediators·T TakanoA V Cybulsky
Mar 29, 2001·Progress in Lipid Research·E A Capper, L A Marshall
May 14, 1999·Trends in Pharmacological Sciences·G Lambeau, M Lazdunski
May 14, 2003·Cellular Signalling·Sajal Chakraborti
Aug 5, 2003·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Bruno L Diaz, Jonathan P Arm
Apr 13, 2000·Biochimica Et Biophysica Acta·H A van der HelmH van den Bosch
Nov 18, 2000·Biochimica Et Biophysica Acta·D A Six, E A Dennis
Nov 18, 2000·Biochimica Et Biophysica Acta·W Cho
Nov 18, 2000·Biochimica Et Biophysica Acta·S YedgarE Schnitzer
Sep 9, 1999·Biochimica Et Biophysica Acta·M J JanssenM R Egmond
Apr 28, 2006·Journal of Medicinal Chemistry·Daren StephensGeorge Kokotos
Sep 1, 1999·European Journal of Biochemistry·H SawadaI Kudo
Mar 18, 2000·Kidney International·A V CybulskyA J McTavish

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Antisense Oligonucleotides: ND

This feed focuses on antisense oligonucleotide therapies such as Inotersen, Nusinursen, and Patisiran, in neurodegenerative diseases including amyotrophic lateral sclerosis.