Novel hairpin-shaped primer assay to study the association of the -44 single-nucleotide polymorphism of the DEFB1 gene with early-onset periodontal disease

Clinical and Diagnostic Laboratory Immunology
Michele BoniottoGrant Gallagher

Abstract

A powerful, cost-effective new method for studying single-nucleotide polymorphisms (SNPs) is described. This method is based on the use of hairpin-shaped primers (HP), which give a sensitive and specific PCR amplification of each specific allele, without the use of costly fluorophore-labeled probes and any post-PCR manipulation. The amplification is monitored in real-time using SYBR Green I dye and takes only 2 h to yield results. The HP assay has a simple design and utilizes a conventional real-time PCR apparatus. The -44 C-->G transversion in the DEFB1 gene (which encodes human beta-defensin 1) has been previously associated with Candida carriage in oral epithelia. In this study, we analyzed the association between early-onset periodontal disease (EOP) and the -44 SNP. We used an HP assay to study the distribution of the -44 SNP in 264 human DNAs obtained from two cohorts of EOP patients and healthy controls from different ethnic backgrounds. The results indicate that the -44 SNP has a similar distribution between EOP and healthy patients, suggesting that it is not associated with the disease.

References

Aug 1, 1992·PCR Methods and Applications·R S ChaW G Thilly
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D Y WuR B Wallace
May 1, 1994·Journal of Periodontology·B S Michalowicz
Feb 3, 1998·Nature Biotechnology·S TyagiF R Kramer
Mar 1, 1996·Nature Biotechnology·S Tyagi, F R Kramer
Aug 24, 1999·BioTechniques·L G LeeT M Woudenberg
Sep 27, 2000·Human Molecular Genetics·I C GrayN K Spurr
Mar 29, 2001·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·A DunscheS Jepsen
Dec 1, 2001·Nucleic Acids Research·C M Waterfall, B D Cobb
Feb 7, 2002·Biochemical and Biophysical Research Communications·Ikumi MatsushitaNaoto Keicho
Jun 18, 2002·Infection and Immunity·Catherine A Brissette, Sheila A Lukehart
Jul 27, 2002·Clinical and Experimental Medicine·U HladnikS Crovella
Feb 13, 2004·Mayo Clinic Proceedings·Terence M DohertyRobert C Detrano
Mar 9, 2004·Journal of Clinical Microbiology·Manzour Hernando Hazbón, David Alland
May 1, 1994·Journal of Periodontology·Bryan S Michalowicz

❮ Previous
Next ❯

Citations

Dec 14, 2011·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Marco R KestingMarkus Nieberler
Dec 22, 2009·Future Microbiology·Karl G KohlgrafKim A Brogden
Jun 18, 2009·Pharmacogenomics·María Antonia Sánchez-RomeroAdrián Llerena
Jun 11, 2011·Evidence-based Complementary and Alternative Medicine : ECAM·V F FurlettiJ F Höfling
Jan 10, 2006·Periodontology 2000·Shogo Takashiba, Koji Naruishi
Dec 1, 2012·International Journal of Immunogenetics·R K MehlotraR J Jurevic
Sep 27, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Neil J Gibson
Feb 17, 2015·International Journal of Pediatric Otorhinolaryngology·Fatih ArslanYunus Kasım Terzi
Nov 4, 2006·FEMS Microbiology Letters·Xiaoying LuKim A Brogden
May 31, 2018·Special Care in Dentistry : Official Publication of the American Association of Hospital Dentists, the Academy of Dentistry for the Handicapped, and the American Society for Geriatric Dentistry·Rayanne Soraia Aguiar de Melo DiasRenata Cimões
Nov 17, 2006·International Archives of Allergy and Immunology·Ernesto Prado-Montes de OcaLuis Eduardo Figuera
Apr 8, 2010·Journal of Dental Research·A OzturkA R Vieira
Mar 1, 2012·Human Reproduction·Roger HartJohn P Newnham

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.