Novel helical assembly in arginine methyltransferase 8

Journal of Molecular Biology
Sachiko Toma-FukaiToshiyuki Shimizu

Abstract

Protein arginine methyltransferase 8 (PRMT8) is unique among PRMTs, as it is specifically expressed in brain and localized to the plasma membrane via N-terminal myristoylation. Here, we describe the crystal structure of human PRMT8 (hPRMT8) at 3.0-Å resolution. The crystal structure of hPRMT8 exhibited a novel helical assembly. Biochemical, biophysical and mutagenesis experiments demonstrated that hPRMT8 forms an octamer in solution. This octameric structure is necessary for proper localization to the plasma membrane and efficient methyltransferase activity. The helical assembly might be a relevant quaternary form for hPRMT1, which is the predominant PRMT in mammalian cells and most closely related to hPRMT8.

References

Mar 14, 2000·The Journal of Biological Chemistry·J TangH R Herschman
Oct 10, 2003·Molecular & Cellular Proteomics : MCP·François-Michel BoisvertStéphane Richard
May 1, 1997·Acta Crystallographica. Section D, Biological Crystallography·G N MurshudovE J Dodson
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Feb 17, 2005·Science's STKE : Signal Transduction Knowledge Environment·François-Michel BoisvertStéphane Richard
Mar 23, 2005·Nature Methods·Shao-En OngMatthias Mann
May 5, 2005·Annual Review of Biophysics and Biomolecular Structure·Xiaodong ChengXing Zhang
Jul 30, 2005·The Journal of Biological Chemistry·Jaeho LeeMark T Bedford
Sep 21, 2007·The EMBO Journal·Nathalie Troffer-CharlierJean Cavarelli
Oct 11, 2007·The Journal of Biological Chemistry·Joyce SayeghSteven G Clarke
Jan 20, 2009·Molecular Cell·Mark T Bedford, Steven G Clarke
Aug 1, 2007·Journal of Applied Crystallography·Airlie J McCoyRandy J Read
Jul 9, 2011·Biochemistry·You FengYujun George Zheng
Dec 14, 2012·Nature Reviews. Cancer·Yanzhong Yang, Mark T Bedford
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor

❮ Previous
Next ❯

Citations

Mar 26, 2016·Biochemical and Biophysical Research Communications·Claudia SolariAlejandra Guberman
Sep 20, 2018·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Melody D FultonY George Zheng
May 28, 2019·Cellular and Molecular Life Sciences : CMLS·Sunil Kumar TewaryMeng-Chiao Ho
Dec 22, 2016·The Journal of Biological Chemistry·Lucie KafkováLaurie K Read
Aug 10, 2021·Frontiers in Cell and Developmental Biology·Troy A KervinMichael Overduin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.